caa a22 4500 445339365 CHVBK 20180317142807.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00436-010-2243-x doi (NATIONALLICENCE)springer-10.1007/s00436-010-2243-x Increasing the in vitro proliferation rate of Perkinsus mediterraneus , a parasite of the European flat oyster Ostrea edulis [Elektronische Daten] [Sandra Casas, Yanli Li, Jerome La Peyre] Perkinsus mediterraneus is an alveolate parasite first described in Ostrea edulis from the Balearic Islands (Mediterranean Sea, Spain), and little is known about its biology or the disease it causes. Continuous in vitro cultures of P. mediterraneus have recently been established in the protein-deficient culture medium JL-ODRP-2F to facilitate its study. Parasite proliferation rate in vitro however was low, with densities increasing 2- to 6-fold between subcultures at 6-week intervals. To increase the proliferation rate of P. mediterraneus cultures to rates similar to other Perkinsus species, various culture conditions (temperature, osmolality, pH, O2, and CO2 concentrations), culture procedures (seeding density and frequency of medium changes), concentrations of medium components, and addition of medium supplements (oyster tissue lysate, oyster plasma, animal sera, growth factors, and hormones) were tested. All treatments were evaluated by measuring parasite densities after 2weeks of culture. The greatest increase in parasite densities, a 35-fold increase over the cell seeding density and 18 times that of the control (cells without supplementation), occurred in medium supplemented with 1,000μg/mL of O. edulis tissue lysate. P. mediterraneus proliferation was also significantly increased by oyster tissue lysate concentration as low as 125μg/mL. Springer-Verlag, 2011 Casas Sandra Department of Veterinary Science, Louisiana State University Agricultural Center, 111 Dalrymple Building, 70803, Baton Rouge, LA, USA aut Li Yanli Department of Veterinary Science, Louisiana State University Agricultural Center, 111 Dalrymple Building, 70803, Baton Rouge, LA, USA aut La Peyre Jerome Department of Veterinary Science, Louisiana State University Agricultural Center, 111 Dalrymple Building, 70803, Baton Rouge, LA, USA aut Parasitology Research Springer-Verlag 109/1(2011-07-01), 195-203 0932-0113 109:1<195 2011 109 436 https://doi.org/10.1007/s00436-010-2243-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2243-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Casas Sandra Department of Veterinary Science, Louisiana State University Agricultural Center, 111 Dalrymple Building, 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Li Yanli Department of Veterinary Science, Louisiana State University Agricultural Center, 111 Dalrymple Building, 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- La Peyre Jerome Department of Veterinary Science, Louisiana State University Agricultural Center, 111 Dalrymple Building, 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/1(2011-07-01), 195-203 0932-0113 109:1<195 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer