caa a22 4500 445339373 CHVBK 20180317142807.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00436-010-2239-6 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2239-6 Species delimitation and phylogenetic relationships of Chinese Leishmania isolates reexamined using kinetoplast cytochrome oxidase II gene sequences [Elektronische Daten] [De-Ping Cao, Xian-Guang Guo, Da-Li Chen, Jian-Ping Chen] Leishmaniasis is a geographically widespread disease caused by protozoan parasites belonging to the genus Leishmania and transmitted by certain species of sand fly. This disease still remains endemic in China, especially in the west and northwest frontier regions. A recent ITS1 phylogeny of Chinese Leishmania isolates has challenged some aspects for their traditional taxonomy and cladistic hypotheses of their phylogeny. However, disagreement with respect to relationships within Chinese Leishmania isolates highlights the need for additional data and analyses. Here, we test the phylogenetic relationships among Chinese isolates and their relatives by analyzing kinetoplast cytochrome oxidase II (COII) gene sequences, including 14 Chinese isolates and three isolates from other countries plus 17 sequences retrieved from GenBank. The COII gene might have experienced little substitution saturation, and its evolutionary process was likely to have been stationary, reversible, and homogeneous. Both neighbor-joining and Bayesian analyses reveal a moderately supported group comprising ten newly determined isolates, which is closely related to Leishmania tarentolae and Endotrypanum monterogeii. In combination with genetic distance analysis as well as Bayesian hypothesis testing, this further corroborates the occurrence of an undescribed species of Leishmania. Our results also suggest that (1) isolate MHOM/CN/93/GS7 and isolate IPHL/CN/77/XJ771 are Leishmania donovani; (2) isolate MHOM/CN/84/JS1 is Leishmania tropica; (3) the status referring to an isolate MRHO/CN/62/GS-GER20 from a great gerbil in Gansu, China, as Leishmania gerbilli, formerly based on multilocus enzyme electrophoresis, is recognized; and (4) E. monterogeii is nested within the genus Leishmania, resulting in a paraphyletic Leishmania. In addition, the results of this study enrich our understanding of the heterogeneity and relationships of Chinese Leishmania isolates. Springer-Verlag, 2011 Cao De-Ping Department of Parasitology, West China School of Preclinical and Forensic Medicine, Sichuan University, 610041, Chengdu, Sichuan, China aut Guo Xian-Guang Chengdu Institute of Biology, Chinese Academy of Sciences, 610041, Chengdu, Sichuan, China aut Chen Da-Li Department of Parasitology, West China School of Preclinical and Forensic Medicine, Sichuan University, 610041, Chengdu, Sichuan, China aut Chen Jian-Ping Department of Parasitology, West China School of Preclinical and Forensic Medicine, Sichuan University, 610041, Chengdu, Sichuan, China aut Parasitology Research Springer-Verlag 109/1(2011-07-01), 163-173 0932-0113 109:1<163 2011 109 436 https://doi.org/10.1007/s00436-010-2239-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2239-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cao De-Ping Department of Parasitology, West China School of Preclinical and Forensic Medicine, Sichuan University, 610041, Chengdu, Sichuan, China aut NATIONALLICENCE 700 1- Guo Xian-Guang Chengdu Institute of Biology, Chinese Academy of Sciences, 610041, Chengdu, Sichuan, China aut NATIONALLICENCE 700 1- Chen Da-Li Department of Parasitology, West China School of Preclinical and Forensic Medicine, Sichuan University, 610041, Chengdu, Sichuan, China aut NATIONALLICENCE 700 1- Chen Jian-Ping Department of Parasitology, West China School of Preclinical and Forensic Medicine, Sichuan University, 610041, Chengdu, Sichuan, China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/1(2011-07-01), 163-173 0932-0113 109:1<163 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer