caa a22 4500 44533939X CHVBK 20180317142807.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00436-010-2244-9 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2244-9 Development of a new PCR protocol to detect and subtype Blastocystis spp. from humans and animals [Elektronische Daten] [Mónica Santín, María Gómez-Muñoz, Gloria Solano-Aguilar, Ronald Fayer] Blastocystis spp. is commonly found in the feces of humans worldwide. Infection has been reported as asymptomatic, acute symptomatic, and chronic symptomatic. This wide range of responses to infection could be related to the genetic diversity of morphologically indistinguishable specimens obtained from infected hosts. The former name Blastocystis hominis is now reported as Blastocystis spp. because of its genetic diversity. Blastocystis is recognized as a complex of subtypes that have not been fully characterized as independent species. The finding of Blastocystis spp. in feces from several animal species suggests a zoonotic potential. Based on conserved regions of published nucleotide SSU rDNA sequences from all Blastocystis subtypes found in GenBank, a PCR and sequencing protocol was developed. The ~500bp SSU rDNA gene fragment amplified by this PCR is highly sensitive compared with published primers and contains highly variable regions that allow phylogenetic analysis of Blastocystis. These primers were used to detect and subtype Blastocystis spp. specimens from naturally infected humans, primates, cattle, pigs, and chickens. Based on these findings, application of this method can elucidate the complexity of this heterogeneous genus and its role in human and animal disease, as well as its zoonotic potential. Springer-Verlag (outside the USA), 2011 Santín Mónica Environmental Microbial and Food Safety Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Building 173, BARC-East, 10300 Baltimore Avenue, 20705, Beltsville, MD, USA aut Gómez-Muñoz María Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Avenida Puerta de Hierro S/N, 28040, Madrid, Spain aut Solano-Aguilar Gloria United States Department of Agriculture, Beltsville Nutrition Research Center, Diet Genomics and Immunology Laboratory, Agricultural Research Service, Building 307C, BARC-East, 10300 Baltimore Avenue, 20705, Beltsville, MD, USA aut Fayer Ronald Environmental Microbial and Food Safety Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Building 173, BARC-East, 10300 Baltimore Avenue, 20705, Beltsville, MD, USA aut Parasitology Research Springer-Verlag 109/1(2011-07-01), 205-212 0932-0113 109:1<205 2011 109 436 https://doi.org/10.1007/s00436-010-2244-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2244-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Santín Mónica Environmental Microbial and Food Safety Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Building 173, BARC-East, 10300 Baltimore Avenue, 20705, Beltsville, MD, USA aut NATIONALLICENCE 700 1- Gómez-Muñoz María Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Avenida Puerta de Hierro S/N, 28040, Madrid, Spain aut NATIONALLICENCE 700 1- Solano-Aguilar Gloria United States Department of Agriculture, Beltsville Nutrition Research Center, Diet Genomics and Immunology Laboratory, Agricultural Research Service, Building 307C, BARC-East, 10300 Baltimore Avenue, 20705, Beltsville, MD, USA aut NATIONALLICENCE 700 1- Fayer Ronald Environmental Microbial and Food Safety Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Building 173, BARC-East, 10300 Baltimore Avenue, 20705, Beltsville, MD, USA aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/1(2011-07-01), 205-212 0932-0113 109:1<205 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer