caa a22 4500 445339489 CHVBK 20180317142807.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00436-010-2218-y doi (NATIONALLICENCE)springer-10.1007/s00436-010-2218-y Anthelmintic activity of botanical extracts against sheep gastrointestinal nematodes, Haemonchus contortus [Elektronische Daten] [Chinnaperumal Kamaraj, Abdul Rahuman, Gandhi Elango, Asokan Bagavan, Abdul Zahir] The source of chemical anthelmintics (levamisole, flubendazole, and thiabendazole) had limited the success of gastrointestinal nematodiasis control in sheep and goats and thus awakened interest in the study of medicinal plant extracts as alternative sources of anthelmintics. The egg hatching and larvicidal effect of indigenous plant extracts were investigated against the sheep parasite, Haemonchus contortus. The purpose of the present study was to assess the efficacy of leaf, bark, and seed ethyl acetate, acetone and methanol extracts of Andrographis paniculata (Burm.f.) Wall. ex Nees., Anisomeles malabarica (L.) R. Br., Annona squamosa L., Datura metel L., and Solanum torvum Swartz were tested against the parasitic nematode of small ruminants H. contortus using egg hatch assay (EHA) and larval development assay (LDA). The assays were run in 24-well cell culture plates at room temperature with five replicates. All plant extracts showed moderate parasitic effects after 48 and exposure for egg hatching and LDA, respectively; however, 100% egg hatching and larvicidal inhibition were found in the methanol extracts of A. paniculata, A. squamosa, D. metel, and S. torvum at 25 mg/ml and the effect was similar to positive control of Albendazole (0.075 mg/ml) and Ivermectin (0.025mg/ml) against H. contortus, respectively. The EHA result showed the ED50 of methanol extracts of A. paniculata and D. metel, which were 2.90 and 3.08 mg/ml, and in larval development assay, the ED50 was 4.26and 3.86 mg/ml, respectively. These effects remain to be confirmed through in vivo studies. Springer-Verlag, 2010 Kamaraj Chinnaperumal Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, 632 509, Vellore, Tamil Nadu, India aut Rahuman Abdul Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, 632 509, Vellore, Tamil Nadu, India aut Elango Gandhi Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, 632 509, Vellore, Tamil Nadu, India aut Bagavan Asokan Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, 632 509, Vellore, Tamil Nadu, India aut Zahir Abdul Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, 632 509, Vellore, Tamil Nadu, India aut Parasitology Research Springer-Verlag 109/1(2011-07-01), 37-45 0932-0113 109:1<37 2011 109 436 https://doi.org/10.1007/s00436-010-2218-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2218-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kamaraj Chinnaperumal Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, 632 509, Vellore, Tamil Nadu, India aut NATIONALLICENCE 700 1- Rahuman Abdul Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, 632 509, Vellore, Tamil Nadu, India aut NATIONALLICENCE 700 1- Elango Gandhi Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, 632 509, Vellore, Tamil Nadu, India aut NATIONALLICENCE 700 1- Bagavan Asokan Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, 632 509, Vellore, Tamil Nadu, India aut NATIONALLICENCE 700 1- Zahir Abdul Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, 632 509, Vellore, Tamil Nadu, India aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/1(2011-07-01), 37-45 0932-0113 109:1<37 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer