caa a22 4500 445339500 CHVBK 20180317142807.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00436-010-2236-9 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2236-9 Prevalence of molecular markers of Plasmodium falciparum resistance to sulfadoxine-pyrimethamine during the intermittent preventive treatment in infants coupled with the expanded program immunization in Senegal [Elektronische Daten] [Babacar Faye, Magatte Ndiaye, Jean Ndiaye, Abiola Annie, Roger Tine, Aminata Lo, Mohamed Ndiaye, Doudou Sow, Alexandra De Sousa, Oumar Gaye] Several studies have shown the efficacy of the intermittent preventive treatment (IPT) using sulfadoxine-pyrimethamine (SP) coupled with the expanded program of immunization (EPI) in infants. However, its adoption as a strategy is conditioned by the long-term efficacy of SP. The impact of IPT-SP coupled with the EPI on the prevalence of markers of resistance to SP was evaluated during this study conducted in Southern Senegal. Three cross-sectional surveys in two health districts (IPT+) were conducted prior to the implementation, 1year, and 2years after. A third district located between the two districts served as a test zone (IPT−). PCR tests were carried out from filter papers collected in children under five for the two first measures and from positive rapid diagnostic tests in the same population for the third measure. Mutations in codons 51, 59, and 108 of the DHFR gene and in codons 437 and 540 of the DHPS were analyzed. The results showed that the prevalence of DHFR triple mutation was more frequent after 2years in IPT+ areas. Regarding quadruple mutation, DHFR (51, 59, and 108) and DHPS (437), no difference was noted between the two areas. The quintuple mutation was not observed after 2years of implementation in both areas. However, an individual analysis showed significant differences in the individual mutation points 51, 59, 108, and 437. This study reveals that despite an increase in the prevalence of individual mutations, the IPT-SP coupled with the EPI has no major impact on DHFR and DHPS combined mutations. Springer-Verlag, 2010 Faye Babacar Department of Parasitology, Faculty of de Medicine, Cheikh Anta Diop University of Dakar, Dakar, Senegal aut Ndiaye Magatte Department of Parasitology, Faculty of de Medicine, Cheikh Anta Diop University of Dakar, Dakar, Senegal aut Ndiaye Jean Department of Parasitology, Faculty of de Medicine, Cheikh Anta Diop University of Dakar, Dakar, Senegal aut Annie Abiola Department of Parasitology, Faculty of de Medicine, Cheikh Anta Diop University of Dakar, Dakar, Senegal aut Tine Roger Department of Parasitology, Faculty of de Medicine, Cheikh Anta Diop University of Dakar, Dakar, Senegal aut Lo Aminata Department of Parasitology, Faculty of de Medicine, Cheikh Anta Diop University of Dakar, Dakar, Senegal aut Ndiaye Mohamed Department of Parasitology, Faculty of de Medicine, Cheikh Anta Diop University of Dakar, Dakar, Senegal aut Sow Doudou Department of Parasitology, Faculty of de Medicine, Cheikh Anta Diop University of Dakar, Dakar, Senegal aut De Sousa Alexandra Unit of Policy and Evidence, Health Section, UNICEF New York, 3 United Nations Plaza, 10017, New York, NY, USA aut Gaye Oumar Department of Parasitology, Faculty of de Medicine, Cheikh Anta Diop University of Dakar, Dakar, Senegal aut Parasitology Research Springer-Verlag 109/1(2011-07-01), 133-138 0932-0113 109:1<133 2011 109 436 https://doi.org/10.1007/s00436-010-2236-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2236-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Faye Babacar Department of Parasitology, Faculty of de Medicine, Cheikh Anta Diop University of Dakar, Dakar, Senegal aut NATIONALLICENCE 700 1- Ndiaye Magatte Department of Parasitology, Faculty of de Medicine, Cheikh Anta Diop University of Dakar, Dakar, Senegal aut NATIONALLICENCE 700 1- Ndiaye Jean Department of Parasitology, Faculty of de Medicine, Cheikh Anta Diop University of Dakar, Dakar, Senegal aut NATIONALLICENCE 700 1- Annie Abiola Department of Parasitology, Faculty of de Medicine, Cheikh Anta Diop University of Dakar, Dakar, Senegal aut NATIONALLICENCE 700 1- Tine Roger Department of Parasitology, Faculty of de Medicine, Cheikh Anta Diop University of Dakar, Dakar, Senegal aut NATIONALLICENCE 700 1- Lo Aminata Department of Parasitology, Faculty of de Medicine, Cheikh Anta Diop University of Dakar, Dakar, Senegal aut NATIONALLICENCE 700 1- Ndiaye Mohamed Department of Parasitology, Faculty of de Medicine, Cheikh Anta Diop University of Dakar, Dakar, Senegal aut NATIONALLICENCE 700 1- Sow Doudou Department of Parasitology, Faculty of de Medicine, Cheikh Anta Diop University of Dakar, Dakar, Senegal aut NATIONALLICENCE 700 1- De Sousa Alexandra Unit of Policy and Evidence, Health Section, UNICEF New York, 3 United Nations Plaza, 10017, New York, NY, USA aut NATIONALLICENCE 700 1- Gaye Oumar Department of Parasitology, Faculty of de Medicine, Cheikh Anta Diop University of Dakar, Dakar, Senegal aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/1(2011-07-01), 133-138 0932-0113 109:1<133 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer