caa a22 4500 445339594 CHVBK 20180317142807.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00436-011-2320-9 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2320-9 Activity of the chelerythrine, a quaternary benzo[ c ]phenanthridine alkaloid from Chelidonium majus L [Elektronische Daten] on Dactylogyrus intermedius [Xi-Lian Li, Jia-Yun Yao, Zhi-Ming Zhou, Jin-Yu Shen, Hong-shun Ru, Xiao-Lin Liu] Dactylogyrus intermedius is a significant monogenean parasite on the gills of cyprinid fishes and can cause severe economic losses in aquaculture and ornamental fish breeding. In the present study, bioactivity-guide fractionation was employed to identify active compound from Chelidonium majus L. against D. intermedius. In vivo anthelmintic activity of petroleum ether, ethyl acetate, chloroform, and n-butanol extracts of C. majus were tested. Among them, only the n-butanol extract exhibited promising anthelmintic efficacy, and therefore subjected to the further isolation and purification using various chromatographic techniques. A compound showing potent activity was obtained and identified by hydrogen, carbon-13 nuclear magnetic resonance spectrum and electron ionization mass spectrometry as chelerythrine. In vivo anthelmintic efficacy tests exhibited that chelerythrine was 100% effective against D. intermedius at a concentration of 1.60mgL−1, with LC50 values of 0.68mgL−1 after 48h of exposure. The 48-h LC50 value (acute toxicity tests) of chelerythrine was found to be 3.59mgL−1 for grass carp. These results provided evidence that chelerythrine can be selected as a lead compound for the development of new drugs against D. intermedius. Springer-Verlag, 2011 Li Xi-Lian College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China aut Yao Jia-Yun Institute of Freshwater Fisheries of Zhejiang, Huzhou, 313001, Zhejiang, China aut Zhou Zhi-Ming Institute of Freshwater Fisheries of Zhejiang, Huzhou, 313001, Zhejiang, China aut Shen Jin-Yu Institute of Freshwater Fisheries of Zhejiang, Huzhou, 313001, Zhejiang, China aut Ru Hong-shun Institute of Freshwater Fisheries of Zhejiang, Huzhou, 313001, Zhejiang, China aut Liu Xiao-Lin College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China aut Parasitology Research Springer-Verlag 109/1(2011-07-01), 247-252 0932-0113 109:1<247 2011 109 436 https://doi.org/10.1007/s00436-011-2320-9 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2320-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Xi-Lian College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China aut NATIONALLICENCE 700 1- Yao Jia-Yun Institute of Freshwater Fisheries of Zhejiang, Huzhou, 313001, Zhejiang, China aut NATIONALLICENCE 700 1- Zhou Zhi-Ming Institute of Freshwater Fisheries of Zhejiang, Huzhou, 313001, Zhejiang, China aut NATIONALLICENCE 700 1- Shen Jin-Yu Institute of Freshwater Fisheries of Zhejiang, Huzhou, 313001, Zhejiang, China aut NATIONALLICENCE 700 1- Ru Hong-shun Institute of Freshwater Fisheries of Zhejiang, Huzhou, 313001, Zhejiang, China aut NATIONALLICENCE 700 1- Liu Xiao-Lin College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/1(2011-07-01), 247-252 0932-0113 109:1<247 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer