caa a22 4500 445339608 CHVBK 20180317142807.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00436-010-2222-2 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2222-2 Involvement of the GP63 protease in infection of Trichomonas vaginalis [Elektronische Daten] [Lina Ma, Qingshu Meng, Weihung Cheng, Yunju Sung, Petrus Tang, Songnian Hu, Jun Yu] There are 48 members of the GP63 protease family in Trichomonas vaginalis according to our annotations; 37 of them are predicted to be transmembrane proteins. Because the GP63 protease family is the largest surface protease family and the second largest surface protein family, they are most likely to be involved in the interactions between T. vaginalis and the host cell surfaces, or otherwise participate in infection. We performed a preliminary study on the functions of GP63 in T. vaginalis (TvGP63). We demonstrated the cell surface localization of one highly expressed member of TvGP63 using indirect immunofluorescence assays in both isolate T016 and isolate 30236. The specific inhibitor of TvGP63 protease, 1,10-phenanthroline, was found to significantly inhibit the destruction of HeLa cells, whereas another chelator, EDTA, could not. Further tests showed that 1,10-phenanthroline did not inhibit the adherence of T. vaginalis cells to HeLa cells. The results presented in here suggest that GP63 protease plays a vital role in T. vaginalis infection process, but may not be related to the adherence of parasitic cells to their hosts. Springer-Verlag, 2011 Ma Lina CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, No.7 Beitucheng West Road, Chaoyang District, 100029, Beijing, People's Republic of China aut Meng Qingshu CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, No.7 Beitucheng West Road, Chaoyang District, 100029, Beijing, People's Republic of China aut Cheng Weihung Molecular Regulation and Bioinformatics Laboratory, Chang Gung University, 333, Taoyuan, Taiwan aut Sung Yunju Institute of Microbiology and Immunology, Medical College, 70101, National Cheng Kung, Taiwan aut Tang Petrus Molecular Regulation and Bioinformatics Laboratory, Chang Gung University, 333, Taoyuan, Taiwan aut Hu Songnian CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, No.7 Beitucheng West Road, Chaoyang District, 100029, Beijing, People's Republic of China aut Yu Jun CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, No.7 Beitucheng West Road, Chaoyang District, 100029, Beijing, People's Republic of China aut Parasitology Research Springer-Verlag 109/1(2011-07-01), 71-79 0932-0113 109:1<71 2011 109 436 https://doi.org/10.1007/s00436-010-2222-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2222-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ma Lina CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, No.7 Beitucheng West Road, Chaoyang District, 100029, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Meng Qingshu CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, No.7 Beitucheng West Road, Chaoyang District, 100029, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Cheng Weihung Molecular Regulation and Bioinformatics Laboratory, Chang Gung University, 333, Taoyuan, Taiwan aut NATIONALLICENCE 700 1- Sung Yunju Institute of Microbiology and Immunology, Medical College, 70101, National Cheng Kung, Taiwan aut NATIONALLICENCE 700 1- Tang Petrus Molecular Regulation and Bioinformatics Laboratory, Chang Gung University, 333, Taoyuan, Taiwan aut NATIONALLICENCE 700 1- Hu Songnian CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, No.7 Beitucheng West Road, Chaoyang District, 100029, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Yu Jun CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, No.7 Beitucheng West Road, Chaoyang District, 100029, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/1(2011-07-01), 71-79 0932-0113 109:1<71 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer