caa a22 4500 445339659 CHVBK 20180317142808.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00436-011-2443-z doi (NATIONALLICENCE)springer-10.1007/s00436-011-2443-z Cloning and characterization of ribosomal protein S29, a deltamethrin resistance associated gene from Culex pipiens pallens [Elektronische Daten] [Haibo Sun, Linchun Sun, Ji He, Bo Shen, Jing Yu, Chen Chen, Mifang Yang, Yan Sun, Donghui Zhang, Lei Ma, Changliang Zhu] The 40S ribosomal protein S29 (RPS29) was shown differentially expressed in deltamethrin (DM)-susceptible and resistant Culex pipiens pallens previously. Here, we cloned RPS29 from this mosquito. An open reading frame (ORF) of 171bp was found to encode a putative protein with 56 amino acid residues, which shares more than 90% identities with other mosquito RPS29 genes. Moreover, quantitative real-time PCR analysis demonstrated that the transcriptional level of RPS29 was significantly up-regulated both in DM-resistant Cx. p. pallens and C6/36 cells compared with the susceptible ones. Over-expression of RPS29 showed a slight decrease of cell viability in DM-susceptible C6/36 cells under DM treatment and knockdown of RPS29 by siRNA comparably increased cell viability in DM-resistant cells. Our study provides evidence that RPS29 might be associated with fitness cost of DM resistance in mosquitoes for the first time. Springer-Verlag, 2011 Sun Haibo Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut Sun Linchun Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut He Ji State Key Laboratory of Monitoring and Detection for Medical Vectors, Xiamen Entry-Exit Inspection and Quarantine Bureau, 361012, Xiamen, Fujian, People's Republic of China aut Shen Bo Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut Yu Jing Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut Chen Chen Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut Yang Mifang Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut Sun Yan Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut Zhang Donghui Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut Ma Lei Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut Zhu Changliang Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut Parasitology Research Springer-Verlag 109/6(2011-12-01), 1689-1697 0932-0113 109:6<1689 2011 109 436 https://doi.org/10.1007/s00436-011-2443-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2443-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sun Haibo Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Sun Linchun Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- He Ji State Key Laboratory of Monitoring and Detection for Medical Vectors, Xiamen Entry-Exit Inspection and Quarantine Bureau, 361012, Xiamen, Fujian, People's Republic of China aut NATIONALLICENCE 700 1- Shen Bo Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Yu Jing Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Chen Chen Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Yang Mifang Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Sun Yan Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Donghui Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Ma Lei Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Zhu Changliang Department of Pathogen Biology, Nanjing Medical University, 210029, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/6(2011-12-01), 1689-1697 0932-0113 109:6<1689 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer