caa a22 4500 445339675 CHVBK 20180317142808.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00436-011-2432-2 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2432-2 The intraperitoneal inoculation of Lactobacillus casei in mice induces total protection against Trichinella spiralis infection at low challenge doses [Elektronische Daten] [Federico Martínez-Gómez, Beatriz Fuentes-Castro, Carlos Bautista-Garfias] The following effects of Lactobacillus casei in NIH mice were evaluated: the establishment of Trichinella spiralis adult worms in the intestine (AWI), larvae per gram of muscle tissue (LPG), levels of IgG and IgA, and levels of IL-4 and IFN-γ. One hundred and eight mice were allocated at random into 18 groups of six mice each. Each mouse in treated or non-treated groups was inoculated intraperitoneally once a week during 6weeks with L. casei or phosphate-buffered solution. Later each mouse was challenged either with 200, 50, or 25 T. spiralis infective larvae. When the infection dose was 200 T. spiralis infective larvae, the reductions in AWI were 78.6% at 4days after infection (dai) and 76.7% at 10dai; while the reduction of LPG was 80.9% with respect to control groups. When the infection dose was 50 or 25 T. spiralis infective larvae, the reductions of AWI were 100% both at 4 and 10dai; while the reduction of LPG at 30dai was also 100% with respect to control groups. The levels of IgG and IgA anti-T. spiralis and IL-4 were significantly higher (P < 0.01) at 4 and 10dai in mice from groups treated with L. casei than in animals in control groups; while at 10dai, the levels of IFN-γ were higher in control mice (P < 0.01) than in L. casei-treated animals. The results suggest that frequent treatment of mice with L. casei induces a total protection against infection with low doses of T. spiralis. Springer-Verlag, 2011 Martínez-Gómez Federico Laboratorio de Inmunoparasitología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala, Col. Santo Tomás, C.P. 11340, Mexico, DF, Mexico aut Fuentes-Castro Beatriz Laboratorio de Inmunoparasitología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala, Col. Santo Tomás, C.P. 11340, Mexico, DF, Mexico aut Bautista-Garfias Carlos Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Carr. Fed. Cuernavaca-Cuautla no. 8534, Col. Progreso, P.P. 206, CIVAC, C.P. 62550, Jiutepec, Morelos, Mexico aut Parasitology Research Springer-Verlag 109/6(2011-12-01), 1609-1617 0932-0113 109:6<1609 2011 109 436 https://doi.org/10.1007/s00436-011-2432-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2432-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Martínez-Gómez Federico Laboratorio de Inmunoparasitología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala, Col. Santo Tomás, C.P. 11340, Mexico, DF, Mexico aut NATIONALLICENCE 700 1- Fuentes-Castro Beatriz Laboratorio de Inmunoparasitología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala, Col. Santo Tomás, C.P. 11340, Mexico, DF, Mexico aut NATIONALLICENCE 700 1- Bautista-Garfias Carlos Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Carr. Fed. Cuernavaca-Cuautla no. 8534, Col. Progreso, P.P. 206, CIVAC, C.P. 62550, Jiutepec, Morelos, Mexico aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/6(2011-12-01), 1609-1617 0932-0113 109:6<1609 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer