caa a22 4500 44533973X CHVBK 20180317142808.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00436-011-2442-0 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2442-0 Cryptosporidium parvum oocyst viability and behaviour of the residual body during the excystation process [Elektronische Daten] [Sirri Kar, Arwid Daugschies, Ayse Cakmak, Nadim Yilmazer, Katja Dittmar, Berit Bangoura] This study was conducted as a comparative evaluation of time-dependent changes in the viability of purified Cryptosporidium parvum oocysts by means of different excystation methods. Oocyst samples were 2weeks to 12months old and were treated with bile or sodium taurocholate, partly after pretreatment with hypochlorite. Pretreatment markedly enhanced the excystation of younger oocyst samples but did not increase excystation rates of 9 or 12-month-old oocysts. A cell culture-PCR assay was used as a second indicator for oocyst viability and was most consistent with excystation trials including oocyst pretreatment. In experiments aiming at the determination of the behaviour of the oocyst residual body during excystation, it could be demonstrated that it might be involved in this process. Springer-Verlag, 2011 Kar Sirri Department of Biology, Namik Kemal University, 59030, Tekirdag, Turkey aut Daugschies Arwid Institute of Parasitology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 35, 04103, Leipzig, Germany aut Cakmak Ayse Department of Parasitology, Faculty of Veterinary Medicine, University of Ankara, 06110, Ankara, Turkey aut Yilmazer Nadim Department of Biology, Namik Kemal University, 59030, Tekirdag, Turkey aut Dittmar Katja Institute of Parasitology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 35, 04103, Leipzig, Germany aut Bangoura Berit Institute of Parasitology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 35, 04103, Leipzig, Germany aut Parasitology Research Springer-Verlag 109/6(2011-12-01), 1719-1723 0932-0113 109:6<1719 2011 109 436 https://doi.org/10.1007/s00436-011-2442-0 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2442-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kar Sirri Department of Biology, Namik Kemal University, 59030, Tekirdag, Turkey aut NATIONALLICENCE 700 1- Daugschies Arwid Institute of Parasitology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 35, 04103, Leipzig, Germany aut NATIONALLICENCE 700 1- Cakmak Ayse Department of Parasitology, Faculty of Veterinary Medicine, University of Ankara, 06110, Ankara, Turkey aut NATIONALLICENCE 700 1- Yilmazer Nadim Department of Biology, Namik Kemal University, 59030, Tekirdag, Turkey aut NATIONALLICENCE 700 1- Dittmar Katja Institute of Parasitology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 35, 04103, Leipzig, Germany aut NATIONALLICENCE 700 1- Bangoura Berit Institute of Parasitology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 35, 04103, Leipzig, Germany aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/6(2011-12-01), 1719-1723 0932-0113 109:6<1719 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer