caa a22 4500 445339764 CHVBK 20190328074407.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00436-011-2418-0 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2418-0 In vitro trematocidal effects of crude alcoholic extracts of Artemisia annua , A. absinthium , Asimina triloba , and Fumaria officinalis [Elektronische Daten] Trematocidal plant alcoholic extracts [Jorge Ferreira, Paul Peaden, Jennifer Keiser] Trematode infections negatively affect human and livestock health, and threaten global food safety. The only approved human anthelmintics for trematodiasis are triclabendazole and praziquantel with no alternative drugs in sight. We tested six crude plant extracts against adult Schistosoma mansoni, Fasciola hepatica, and Echinostoma caproni in vitro. Mortality was best achieved by ethanolic extracts of Artemisia annua (sweet Annie), Asimina triloba (paw-paw), and Artemisia absinthium (wormwood) which, at 2mg/mL, killed S. mansoni and E. caproni in 20h or less (except for wormwood), F. hepatica between 16 and 23h (sweet Annie), or 40h (paw-paw). Some extracts were active at 0.2mg/mL and 20μg/mL, although more time was required to kill trematodes. However, aqueous A. annua and methanol extracts of Fumaria officinalis had no activity. Chromatographic analysis of the three best extracts established that A. annua and A. triloba extracts contained bioactive artemisinin and acetogenins (asimicin and bullatacin), respectively. The anthelmintic activity of our extracts at such low doses indicates that their anthelmintic activity deserves further testing as natural alternative controls for parasites of both animals and humans. Our results also support recent evidence that synergistic effects of multiple bioactive compounds present in crude plant extracts is worth exploring. Springer-Verlag (outside the USA), 2011 Ferreira Jorge USDA-ARS, Appalachian Farming Systems Research Center, 1224 Airport Rd., 25813, Beaver, WV, USA aut Peaden Paul Nature Sunshine Products, 1655 North Main St., 84660, Spanish Fork, UT, USA aut Keiser Jennifer Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, CH-4003, Basel, Switzerland aut Parasitology Research Springer-Verlag 109/6(2011-12-01), 1585-1592 0932-0113 109:6<1585 2011 109 436 https://doi.org/10.1007/s00436-011-2418-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2418-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ferreira Jorge USDA-ARS, Appalachian Farming Systems Research Center, 1224 Airport Rd., 25813, Beaver, WV, USA aut NATIONALLICENCE 700 1- Peaden Paul Nature Sunshine Products, 1655 North Main St., 84660, Spanish Fork, UT, USA aut NATIONALLICENCE 700 1- Keiser Jennifer Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, CH-4003, Basel, Switzerland aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/6(2011-12-01), 1585-1592 0932-0113 109:6<1585 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer