caa a22 4500 445339802 CHVBK 20180317142808.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00436-011-2437-x doi (NATIONALLICENCE)springer-10.1007/s00436-011-2437-x Genetic polymorphism at the C-terminal domain (region III) of knob-associated histidine-rich protein (KAHRP) of Plasmodium falciparum in isolates from Iran [Elektronische Daten] [Ahmad Mardani, Hossein Keshavarz, Aliehsan Heidari, Homa Hajjaran, Ahmad Raeisi, Mohammad Khorramizadeh] The knob-associated histidine-rich protein (KAHRP) plays a major role in the virulence of Plasmodium falciparum and is one of the targets for molecular therapy. The primary structure of KAHRP of P. falciparum consists of three domains (regions I-III), of which the C-terminal domain (region III) is the most polymorphic segment of this protein. One of the main obstacles is genetic diversity in designing and developing of malaria control strategies such as molecular therapy and vaccines. The primary objective of the present study was to investigate and analyze the extent of genetic polymorphism at the region III of KAHRP of P. falciparum in isolates from Iran. A fragment of the kahrp gene spanning the C-terminal domain was amplified by nested PCR from 50 P. falciparum isolates collected from two malaria endemic areas of Iran during 2009 to August 2010 and sequenced. In this study, three allelic types were observed at the C-terminal domain of KAHRP on the basis of the molecular weight of nested PCR products and the obtained sequencing data. The presence of multiple alleles of the kahrp gene indicates that several P. falciparum strains exist in the malaria endemic areas of Iran. Our findings will be valuable in the design and the development of the molecular therapeutic reagents for falciparum malaria. Springer-Verlag, 2011 Mardani Ahmad Department of Medical Parasitology and Mycology, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences (TUMS), P.O. Box14155-6446, Tehran, Iran aut Keshavarz Hossein Department of Medical Parasitology and Mycology, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences (TUMS), P.O. Box14155-6446, Tehran, Iran aut Heidari Aliehsan Department of Medical Sciences, Iran Encyclopedia Compiling Foundation, Ministry of Sciences, Tehran, Iran aut Hajjaran Homa Department of Medical Parasitology and Mycology, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences (TUMS), P.O. Box14155-6446, Tehran, Iran aut Raeisi Ahmad Department of Medical Entomology and Vector Control, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences (TUMS), Tehran, Iran aut Khorramizadeh Mohammad Department of Medical Biotechnology, School of Advanced Medical Technologies, Tehran University of Medical Sciences (TUMS), Tehran, Iran aut Parasitology Research Springer-Verlag 109/6(2011-12-01), 1647-1652 0932-0113 109:6<1647 2011 109 436 https://doi.org/10.1007/s00436-011-2437-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2437-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mardani Ahmad Department of Medical Parasitology and Mycology, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences (TUMS), P.O. Box14155-6446, Tehran, Iran aut NATIONALLICENCE 700 1- Keshavarz Hossein Department of Medical Parasitology and Mycology, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences (TUMS), P.O. Box14155-6446, Tehran, Iran aut NATIONALLICENCE 700 1- Heidari Aliehsan Department of Medical Sciences, Iran Encyclopedia Compiling Foundation, Ministry of Sciences, Tehran, Iran aut NATIONALLICENCE 700 1- Hajjaran Homa Department of Medical Parasitology and Mycology, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences (TUMS), P.O. Box14155-6446, Tehran, Iran aut NATIONALLICENCE 700 1- Raeisi Ahmad Department of Medical Entomology and Vector Control, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences (TUMS), Tehran, Iran aut NATIONALLICENCE 700 1- Khorramizadeh Mohammad Department of Medical Biotechnology, School of Advanced Medical Technologies, Tehran University of Medical Sciences (TUMS), Tehran, Iran aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/6(2011-12-01), 1647-1652 0932-0113 109:6<1647 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer