caa a22 4500 445339810 CHVBK 20180317142808.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00436-011-2440-2 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2440-2 Larvicidal activities of Knema attenuata (Hook. f. & Thomson) Warb. (Myristicaceae) extracts against Aedes albopictus Skuse and Anopheles stephensi Liston [Elektronische Daten] [Vinayachandra,, R. Shwetha, K. Chandrashekar] In recent years, uses of environment friendly and biodegradable natural insecticides of plant origin have received renewed attention as agents for vector control. The present study was undertaken to investigate the effect of aril and kernel extracts of Knema attenuata (Hook. f. & Thomson) Warb. (Myristicaceae) on larvae of Aedes albopictus Skuse and Anopheles stephensi Liston under laboratory conditions. The aril was extracted with chloroform and ethanol; the kernel was extracted with ethanol and hexane. The extracts were tested against the 3rd-4th instar larvae collected from Bunder area, Mangalore, India, which is a well-known fishing harbour, where several mosquito-borne diseases were reported. All the graded concentrations (100, 200, 300, 400 and 500ppm) showed significant larval mortality after 24h of observation. Chloroform extracts of aril showed 100% mortality against both larval forms of A. albopictus and A. stephensi at the concentration of 500ppm. Among the extracts tested, chloroform extracts of aril and ethanol extracts of kernel exhibited higher toxicity against both A. albopictus (LC50, 141ppm and 159ppm; LC90, 290ppm and 342ppm) and A. stephensi (LC50, 160ppm and 162ppm; LC90, 445ppm and 458ppm). Hexane extracts of kernel exhibited least toxicity against A. albopictus (LC50, 239ppm; LC90, 484ppm), whereas ethanol extracts of aril showed the least toxicity against A. stephensi (LC50, 290; LC90, 498). A preliminary phytochemical assay revealed the presence of phenolics, tannins, steroids, terpenes, resins, and glycolipids in all the extracts. Alkaloids, flavonoids and saponins were absent. The lower LC50 value of the chloroform extracts of K. attenuata aril indicates its potentiality as a larvicide against A. albopictus and A. stephensi mosquito larvae. Springer-Verlag, 2011 Vinayachandra, Department of Applied Botany, Mangalore University, Mangalagangothri, 574199, Mangalore, Karnataka, India aut Shwetha R. Department of Biosciences, Mangalore University, Mangalagangothri, 574199, Mangalore, Karnataka, India aut Chandrashekar K. Department of Applied Botany, Mangalore University, Mangalagangothri, 574199, Mangalore, Karnataka, India aut Parasitology Research Springer-Verlag 109/6(2011-12-01), 1671-1676 0932-0113 109:6<1671 2011 109 436 https://doi.org/10.1007/s00436-011-2440-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2440-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vinayachandra Department of Applied Botany, Mangalore University, Mangalagangothri, 574199, Mangalore, Karnataka, India aut NATIONALLICENCE 700 1- Shwetha R. Department of Biosciences, Mangalore University, Mangalagangothri, 574199, Mangalore, Karnataka, India aut NATIONALLICENCE 700 1- Chandrashekar K. Department of Applied Botany, Mangalore University, Mangalagangothri, 574199, Mangalore, Karnataka, India aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/6(2011-12-01), 1671-1676 0932-0113 109:6<1671 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer