caa a22 4500 445339853 CHVBK 20180317142808.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00436-011-2412-6 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2412-6 Characterization of Sarcocystis fusiformis based on sequencing and PCR-RFLP in water buffalo ( Bubalus bubalis ) in Iran [Elektronische Daten] [Ahmad Oryan, Hassan Sharifiyazdi, Monire Khordadmehr, Sara Larki] Four Sarcocystis species, i.e., Sarcocystis fusiformis and Sarcocystis buffalonis with cats as definitive hosts, Sarcocystis levinei with dogs as definitive host, and Sarcocystis dubeyi with unknown definitive host, have previously been described from water buffalo (Bubalus bubalis). The aim of the present study was genetic characterization of the causative agent(s) of water buffalo sarcocystosis in Khuzestan Province, western Iran. RFLP-PCR and partial sequence analysis of 18S rDNA gene were used for the genetic characterization of the specimens directly obtained from water buffalo. In RFLP-PCR, four restriction enzymes (Dra1, Ssp1, Fok1 and Bsl1) were used for species discrimination of Sarcocystis spp. in this host. Comparison of the molecular sequencing results and RFLP-PCR pattern of the samples obtained in the present study with those previously reported for different Sarcocystis spp. revealed that all positive Sarcocystis samples represented S. fusiformis. To our knowledge, this is the first demonstration of the existence of S. fusiformis in the Iranian water buffalo population by a genetic approach. In addition, comparison between the alignments between the Iranian 18S rDNA sequences (HQ703791), made in this study, and those previously reported for S. fusiformis in different geographical location (accession nos. AF176927, AF176926, and U03071) showed the occurrence of local genetic polymorphisms and heterogeneity in this ribosomal locus. Despite the occurrence of some genetic variations in the hypervariable regions of the 18S rDNA in S. fusiformis, Dra I restriction site was conserved among all sequences available. According to the present study, it seems that cats have a more significant epidemiological role than dogs in transmission of sarcocystosis agent to water buffalo in Iran. Springer-Verlag, 2011 Oryan Ahmad Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran aut Sharifiyazdi Hassan Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran aut Khordadmehr Monire Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran aut Larki Sara Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran aut Parasitology Research Springer-Verlag 109/6(2011-12-01), 1563-1570 0932-0113 109:6<1563 2011 109 436 https://doi.org/10.1007/s00436-011-2412-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2412-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Oryan Ahmad Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran aut NATIONALLICENCE 700 1- Sharifiyazdi Hassan Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran aut NATIONALLICENCE 700 1- Khordadmehr Monire Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran aut NATIONALLICENCE 700 1- Larki Sara Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/6(2011-12-01), 1563-1570 0932-0113 109:6<1563 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer