caa a22 4500 445339888 CHVBK 20180317142808.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00436-011-2441-1 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2441-1 Detection of a morphogenetically novel Sarcocystis hominis -like in the context of a prevalence study in semi-intensively bred cattle in Italy [Elektronische Daten] [Lorenzo Domenis, Simone Peletto, Luciano Sacchi, Emanuela Clementi, Marco Genchi, Lucia Felisari, Carla Felisari, Patrizia Mo, Paola Modesto, Fabio Zuccon, Chiara Campanella, Cristiana Maurella, Cristina Guidetti, Pier Acutis] The aim of this study was to determine the prevalence of sarcosporidiosis in semi-intensively bred cattle in northwestern Italy. A diagnostic protocol was setup in which infected animals were identified by rapid histological examination of the esophagus, diaphragm, and heart and the detected Sarcocystis spp. were subsequently typed using conventional electron microscopy in combination with molecular techniques. Sarcosporidia cysts were detected in 78.1% of the animals and were seen most often in the esophagus. The cattle is intermediate host for Sarcocystis hominis (final host, humans and some primates), Sarcocystis cruzi (final host, domestic and wild canids), and Sarcocystis hirsuta (final host, wild and domestic cats).All these three species of Sarcocystis were identified, variously associated, with the following prevalence: S. cruzi (74.2%), S. hirsuta (1.8%), and S. hominis (42.7%). Furthermore, a new S. hominis-like (prevalence 18.5%), characterized by hook-like structures of villar protrusion and a different sequence of the 18S rRNA gene, was identified. The cattle sheds testing positive for zoonotic Sarcocystis were assessed for risk factors contributing to the maintenance of the parasite's life cycle. Significant associations emerged between consumption of raw meat by the farm owner, mountain pasturing, and absence of a sewerage system on the farm and cattle breed. Our study demonstrates that sarcosporidiosis may constitute a public health problem in Italy and indicates several issues to be addressed when planning surveillance and prevention actions. The applied diagnostic approach revealed that cattle can harbor a further type of Sarcocystis, of which life cycle and zoonotic potential should be investigated. Springer-Verlag, 2011 Domenis Lorenzo Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy aut Peletto Simone Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy aut Sacchi Luciano Dipartimento di Biologia Animale, Università di Pavia, Via Ferrata 1, 27100, Pavia, Italy aut Clementi Emanuela Dipartimento di Biologia Animale, Università di Pavia, Via Ferrata 1, 27100, Pavia, Italy aut Genchi Marco Dipartimento di Biologia Animale, Università di Pavia, Via Ferrata 1, 27100, Pavia, Italy aut Felisari Lucia Azienda Sanitaria Locale Biella, Struttura Semplice Area B, Via Don Sturzo 20, 13900, Biella, Italy aut Felisari Carla Azienda Sanitaria Locale Biella, Struttura Semplice Area B, Via Don Sturzo 20, 13900, Biella, Italy aut Mo Patrizia Azienda Sanitaria Locale Biella, Struttura Semplice Area B, Via Don Sturzo 20, 13900, Biella, Italy aut Modesto Paola Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy aut Zuccon Fabio Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy aut Campanella Chiara Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy aut Maurella Cristiana Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy aut Guidetti Cristina Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy aut Acutis Pier Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy aut Parasitology Research Springer-Verlag 109/6(2011-12-01), 1677-1687 0932-0113 109:6<1677 2011 109 436 https://doi.org/10.1007/s00436-011-2441-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2441-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Domenis Lorenzo Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy aut NATIONALLICENCE 700 1- Peletto Simone Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy aut NATIONALLICENCE 700 1- Sacchi Luciano Dipartimento di Biologia Animale, Università di Pavia, Via Ferrata 1, 27100, Pavia, Italy aut NATIONALLICENCE 700 1- Clementi Emanuela Dipartimento di Biologia Animale, Università di Pavia, Via Ferrata 1, 27100, Pavia, Italy aut NATIONALLICENCE 700 1- Genchi Marco Dipartimento di Biologia Animale, Università di Pavia, Via Ferrata 1, 27100, Pavia, Italy aut NATIONALLICENCE 700 1- Felisari Lucia Azienda Sanitaria Locale Biella, Struttura Semplice Area B, Via Don Sturzo 20, 13900, Biella, Italy aut NATIONALLICENCE 700 1- Felisari Carla Azienda Sanitaria Locale Biella, Struttura Semplice Area B, Via Don Sturzo 20, 13900, Biella, Italy aut NATIONALLICENCE 700 1- Mo Patrizia Azienda Sanitaria Locale Biella, Struttura Semplice Area B, Via Don Sturzo 20, 13900, Biella, Italy aut NATIONALLICENCE 700 1- Modesto Paola Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy aut NATIONALLICENCE 700 1- Zuccon Fabio Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy aut NATIONALLICENCE 700 1- Campanella Chiara Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy aut NATIONALLICENCE 700 1- Maurella Cristiana Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy aut NATIONALLICENCE 700 1- Guidetti Cristina Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy aut NATIONALLICENCE 700 1- Acutis Pier Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/6(2011-12-01), 1677-1687 0932-0113 109:6<1677 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer