caa a22 4500 445339896 CHVBK 20180317142808.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00436-011-2439-8 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2439-8 The use of high-resolution 1H nuclear magnetic resonance (NMR) spectroscopy in the clinical diagnosis of Acanthamoeba [Elektronische Daten] [Sandra Hauber, Hary Parkes, Ruqaiyyah Siddiqui, Naveed Khan] Acanthamoeba are opportunistic protozoan pathogens that can produce sight-threatening keratitis and fatal granulomatous encephalitis. The successful prognosis requires early diagnosis and differentiation of pathogenic Acanthamoeba spp. followed by aggressive treatment regimen. In this study, we tested the use of high-resolution 1H NMR spectroscopy in the clinical diagnosis of Acanthamoeba. Using NMR spectroscopy combined with Pattern Recognition Analysis (PRA), we analysed variations in the biochemical ‘fingerprint' of invasive and non-invasive Acanthamoeba, its closely related genus, Balamuthia mandrillaris, neuropathogenic Escherichia coli K1 strain E44, a laboratory strain of E. coli K-12, HB101, mammalian cells including human brain microvascular endothelial cells and monkey kidney cells. The findings revealed significant variations in the metabolites of amoebae, mammalian cells and bacteria. Interestingly, 1H NMR spectra provided distinct biochemical profiles of clinical and non-clinical Acanthamoeba isolates highlighting the potential of 1H NMR spectroscopy combined with PRA for the development of a novel diagnostic test that could rapidly identify pathogenic Acanthamoeba isolates with high sensitivity and specificity. Springer-Verlag, 2011 Hauber Sandra School of Biological and Chemical Sciences, Birkbeck College, University of London, London, UK aut Parkes Hary Institute of Neurology, University College London, University of London, London, UK aut Siddiqui Ruqaiyyah Department of Biological and Biomedical Sciences, Aga Khan University, Stadium Road, Karachi, Pakistan aut Khan Naveed Department of Biological and Biomedical Sciences, Aga Khan University, Stadium Road, Karachi, Pakistan aut Parasitology Research Springer-Verlag 109/6(2011-12-01), 1661-1669 0932-0113 109:6<1661 2011 109 436 https://doi.org/10.1007/s00436-011-2439-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2439-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hauber Sandra School of Biological and Chemical Sciences, Birkbeck College, University of London, London, UK aut NATIONALLICENCE 700 1- Parkes Hary Institute of Neurology, University College London, University of London, London, UK aut NATIONALLICENCE 700 1- Siddiqui Ruqaiyyah Department of Biological and Biomedical Sciences, Aga Khan University, Stadium Road, Karachi, Pakistan aut NATIONALLICENCE 700 1- Khan Naveed Department of Biological and Biomedical Sciences, Aga Khan University, Stadium Road, Karachi, Pakistan aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/6(2011-12-01), 1661-1669 0932-0113 109:6<1661 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer