caa a22 4500 445339926 CHVBK 20180317142809.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00436-011-2513-2 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2513-2 Prevalence of Trypanosoma sp. in cattle from Tanzania estimated by conventional PCR and loop-mediated isothermal amplification (LAMP) [Elektronische Daten] [Dusit Laohasinnarong, Oriel Thekisoe, Imna Malele, Boniface Namangala, Akihiro Ishii, Yasuyuki Goto, Shin-ichiro Kawazu, Chihiro Sugimoto, Noboru Inoue] This study compared the prevalence of trypanosome infections estimated by PFR-loop-mediated isothermal amplification (LAMP) with conventional polymerase chain reaction (PCR) tests. One hundred forty eight cattle blood samples were collected from Robanda village, Mara region, Tanzania in April 2008. In conventional PCR, four sets of primers, specific for the detection of Trypanosoma sp., Trypanosoma brucei rhodesiense, Trypanosoma vivax, and Trypanozoon, as well as a modified LAMP were used. Conventional PCR detected no infection or up to 8, 1, and 3 infections with Trypanosoma congolense savannah, Trypanozoon, and T. vivax, respectively, whereas LAMP detected additional 44 Trypanozoon positive cases. Our results clearly indicate that the prevalence of Trypanozoon spp. in cattle in Robanda village estimated by PFR-LAMP (30.4%) was significantly higher than the estimates by PCR assays (0.6-2%). As such, future studies should target epidemiological surveys of Trypanozoon and T. brucei rhodesiense infections in possible reservoir animals by LAMP to further elucidate the actual prevalence of these parasites. Springer-Verlag, 2011 Laohasinnarong Dusit O.I.E. Reference Laboratory on Surra, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, 080-8555, Obihiro, Hokkaido, Japan aut Thekisoe Oriel O.I.E. Reference Laboratory on Surra, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, 080-8555, Obihiro, Hokkaido, Japan aut Malele Imna Tsetse and Trypanosomiasis Research Institute (TTRI), Majani Mapana, Off Korongwe Road, P.O. Box 1026, Tanga, Tanzania aut Namangala Boniface School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, Zambia aut Ishii Akihiro Research Center for Zoonosis Control, Hokkaido University, 060-0818, Sapporo, Hokkaido, Japan aut Goto Yasuyuki O.I.E. Reference Laboratory on Surra, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, 080-8555, Obihiro, Hokkaido, Japan aut Kawazu Shin-ichiro O.I.E. Reference Laboratory on Surra, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, 080-8555, Obihiro, Hokkaido, Japan aut Sugimoto Chihiro Research Center for Zoonosis Control, Hokkaido University, 060-0818, Sapporo, Hokkaido, Japan aut Inoue Noboru O.I.E. Reference Laboratory on Surra, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, 080-8555, Obihiro, Hokkaido, Japan aut Parasitology Research Springer-Verlag 109/6(2011-12-01), 1735-1739 0932-0113 109:6<1735 2011 109 436 https://doi.org/10.1007/s00436-011-2513-2 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2513-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Laohasinnarong Dusit O.I.E. Reference Laboratory on Surra, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, 080-8555, Obihiro, Hokkaido, Japan aut NATIONALLICENCE 700 1- Thekisoe Oriel O.I.E. Reference Laboratory on Surra, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, 080-8555, Obihiro, Hokkaido, Japan aut NATIONALLICENCE 700 1- Malele Imna Tsetse and Trypanosomiasis Research Institute (TTRI), Majani Mapana, Off Korongwe Road, P.O. Box 1026, Tanga, Tanzania aut NATIONALLICENCE 700 1- Namangala Boniface School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, Zambia aut NATIONALLICENCE 700 1- Ishii Akihiro Research Center for Zoonosis Control, Hokkaido University, 060-0818, Sapporo, Hokkaido, Japan aut NATIONALLICENCE 700 1- Goto Yasuyuki O.I.E. Reference Laboratory on Surra, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, 080-8555, Obihiro, Hokkaido, Japan aut NATIONALLICENCE 700 1- Kawazu Shin-ichiro O.I.E. Reference Laboratory on Surra, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, 080-8555, Obihiro, Hokkaido, Japan aut NATIONALLICENCE 700 1- Sugimoto Chihiro Research Center for Zoonosis Control, Hokkaido University, 060-0818, Sapporo, Hokkaido, Japan aut NATIONALLICENCE 700 1- Inoue Noboru O.I.E. Reference Laboratory on Surra, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, 080-8555, Obihiro, Hokkaido, Japan aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/6(2011-12-01), 1735-1739 0932-0113 109:6<1735 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer