caa a22 4500 445339934 CHVBK 20180317142809.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00436-011-2520-3 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2520-3 Microsatellite analysis of Fasciola spp. in Egypt [Elektronische Daten] [Yasser Dar, Said Amer, Bertrand Courtioux, Gilles Dreyfuss] Recently, the topic of diversity in Fasciola population in Egypt is controversial. The present study was performed to study the genetic diversity of isolated flukes based on microsatellites markers. Fasciola worms were collected from different hosts and geographical locations in Egypt. Control samples of Fasciola hepatica from France as well as Fasciola gigantica from Cameroon were included in the study. Collected flukes were identified morphologically and subjected for analysis using four microsatellite markers. Results of microsatellite profile (FM1 and FM2) proved that both species of Fasciola are distributed in Egypt irrespective of geographical location and host. Nevertheless, the microsatellite profile of some analyzed loci (FM2 and FM3) proved that Egyptian flukes showed more alleles compared to the reference ones. Differences of microsatellite profile in Egyptian isolates than that of corresponding reference samples indicate the remarkable diversity of these isolates. The present results highlighted the utility of microsatellite profile to discriminate between Fasciola species and to elucidate the diversity within the species. To our knowledge, this is the first time to study microsatellite polymorphism in Fasciola populations in Egypt. Springer-Verlag, 2011 Dar Yasser Zoology Department, Faculty of Sciences, Tanta University, Tanta, Egypt aut Amer Said Department of Zoology, Faculty of Science, Kafr El-Sheikh University, 33516, Kafr El-Sheikh, Egypt aut Courtioux Bertrand EA 3174 Tropical and Comparative Neuroepidemiology, Institute of Tropical Neurology, IFR 145 GEIST, University of Limoges, Faculty of Pharmacy, Limoges, France aut Dreyfuss Gilles EA 3174 Tropical and Comparative Neuroepidemiology, Institute of Tropical Neurology, IFR 145 GEIST, University of Limoges, Faculty of Pharmacy, Limoges, France aut Parasitology Research Springer-Verlag 109/6(2011-12-01), 1741-1744 0932-0113 109:6<1741 2011 109 436 https://doi.org/10.1007/s00436-011-2520-3 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2520-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dar Yasser Zoology Department, Faculty of Sciences, Tanta University, Tanta, Egypt aut NATIONALLICENCE 700 1- Amer Said Department of Zoology, Faculty of Science, Kafr El-Sheikh University, 33516, Kafr El-Sheikh, Egypt aut NATIONALLICENCE 700 1- Courtioux Bertrand EA 3174 Tropical and Comparative Neuroepidemiology, Institute of Tropical Neurology, IFR 145 GEIST, University of Limoges, Faculty of Pharmacy, Limoges, France aut NATIONALLICENCE 700 1- Dreyfuss Gilles EA 3174 Tropical and Comparative Neuroepidemiology, Institute of Tropical Neurology, IFR 145 GEIST, University of Limoges, Faculty of Pharmacy, Limoges, France aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/6(2011-12-01), 1741-1744 0932-0113 109:6<1741 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer