caa a22 4500 445340053 CHVBK 20180317142809.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00436-011-2314-7 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2314-7 Merozoite release from Plasmodium falciparum -infected erythrocytes involves the transfer of DiIC16 from infected cell membrane to Maurer's clefts [Elektronische Daten] [Gladys Cortés, Maria Caldas, Sonia Rahirant] Merozoite release from infected erythrocytes is a complex process, which is still not fully understood. Such process was characterised at ultra-structural level in this work by labelling erythrocyte membrane with a fluorescent lipid probe and subsequent photo-conversion into an electron-dense precipitate. A lipophilic DiIC16 probe was inserted into the infected erythrocyte surface and the transport of this phospholipid analogue through the erythrocyte membrane was followed up during 48h of the asexual erythrocyte cycle. The lipid probe was transferred from infected erythrocyte membranes to Maurer's clefts during merozoite release, thereby indicating that these membranes remained inside host cells after parasite release. Fluorescent structures were never observed inside infected erythrocytes preceding merozoite exit and merozoites released from infected erythrocyte were not fluorescent. However, specific precipitated material was localised bordering the parasitophorous vacuole membrane and tubovesicular membranes when labelled non-infected erythrocytes were invaded by merozoites. It was revealed that lipids were interchangeable from one membrane to another, passing from infected erythrocyte membrane to Maurer's clefts inside the erythrocyte ghost, even after merozoite release. Maurer's clefts became photo-converted following merozoite release, suggesting that these structures were in close contact with infected erythrocyte membrane during merozoite exit and possibly played some role in malarial parasite exit from the host cell. The Author(s), 2011 Cortés Gladys Cell Biology Group, Public and Tropical Health Department, Faculty of Medicine, Universidad Nacional de Colombia, Bogotá, Colombia aut Caldas Maria Cell Morphology Group, Instituto Nacional de Salud, Av. Calle 26 No. 51-60, Bogotá, Colombia aut Rahirant Sonia Cell Biochemistry Biology Group, Instituto Nacional de Salud, Bogotá, Colombia aut Parasitology Research Springer-Verlag 109/3(2011-09-01), 941-947 0932-0113 109:3<941 2011 109 436 https://doi.org/10.1007/s00436-011-2314-7 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2314-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cortés Gladys Cell Biology Group, Public and Tropical Health Department, Faculty of Medicine, Universidad Nacional de Colombia, Bogotá, Colombia aut NATIONALLICENCE 700 1- Caldas Maria Cell Morphology Group, Instituto Nacional de Salud, Av. Calle 26 No. 51-60, Bogotá, Colombia aut NATIONALLICENCE 700 1- Rahirant Sonia Cell Biochemistry Biology Group, Instituto Nacional de Salud, Bogotá, Colombia aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/3(2011-09-01), 941-947 0932-0113 109:3<941 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer