caa a22 4500 445340061 CHVBK 20180317142809.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00436-011-2285-8 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2285-8 Molecular cloning, characterization, and immunolocalization of two lactate dehydrogenase homologous genes from Taenia solium [Elektronische Daten] [Wuying Du, Fengyu Hu, Yabo Yang, Dong Hu, Xuchu Hu, Xinbing Yu, Jin Xu, Jialin Dai, Xinjiang Liao, Jiang Huang] Two novel genes encoding lactate dehydrogenase A (LDHA) and B (LDHB) homologues, respectively, were identified from the cDNA libraries of adult Taenia solium (T. solium). The two deduced amino acid sequences both show more than 50% identity to the homologues for Danio rerio, Xenopus laevis, Schistosoma japonicum, Sus scrofa, Homo sapiens, et al. The identity of the amino acid sequence between TsLDHA and TsLDHB is 57.4%, and that of the nucleotide sequence is 61.5%. Recombinant TsLDHA homologue (rTsLDHA) and TsLDHB homologue (rTsLDHB) were expressed in Escherichia coli BL21/DE3 and purified. Though there were some differences in the sequence, the two LDH isozyme homologues show similarity in the conserved LDH domain, topological structure, primary immunological traits, localization on the tegument of T. solium adult, and partial physicochemical properties. The linear B-cell epitope analysis of TsLDHA and TsLDHB discovered a TsLDHA specific epitope. The purified rTsLDHA and rTsLDHB could be recognized by rat immuno-sera, serum from swine, or a patient infected with T. solium, respectively, but Western blot analysis showed cross-reactions, not only between these two LDH members but also with other common human tapeworms or helminths. The results suggested that the two LDH homologues are similar in the characteristics of LDH family, and they are not specific antigens for immunodiagnosis. Springer-Verlag, 2011 Du Wuying Department of Parasitology, Guiyang Medical College, Guiyang, China aut Hu Fengyu Institute of Infectious Diseases, Guangzhou No. 8 People's Hospital, Guangzhou, China aut Yang Yabo Department of Dermatology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China aut Hu Dong Institute of Infectious Diseases, Guangzhou No. 8 People's Hospital, Guangzhou, China aut Hu Xuchu Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China aut Yu Xinbing Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China aut Xu Jin Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China aut Dai Jialin Department of Parasitology, Guiyang Medical College, Guiyang, China aut Liao Xinjiang Department of Parasitology, Guiyang Medical College, Guiyang, China aut Huang Jiang Department of Parasitology, Guiyang Medical College, Guiyang, China aut Parasitology Research Springer-Verlag 109/3(2011-09-01), 567-574 0932-0113 109:3<567 2011 109 436 https://doi.org/10.1007/s00436-011-2285-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2285-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Du Wuying Department of Parasitology, Guiyang Medical College, Guiyang, China aut NATIONALLICENCE 700 1- Hu Fengyu Institute of Infectious Diseases, Guangzhou No. 8 People's Hospital, Guangzhou, China aut NATIONALLICENCE 700 1- Yang Yabo Department of Dermatology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China aut NATIONALLICENCE 700 1- Hu Dong Institute of Infectious Diseases, Guangzhou No. 8 People's Hospital, Guangzhou, China aut NATIONALLICENCE 700 1- Hu Xuchu Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China aut NATIONALLICENCE 700 1- Yu Xinbing Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China aut NATIONALLICENCE 700 1- Xu Jin Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China aut NATIONALLICENCE 700 1- Dai Jialin Department of Parasitology, Guiyang Medical College, Guiyang, China aut NATIONALLICENCE 700 1- Liao Xinjiang Department of Parasitology, Guiyang Medical College, Guiyang, China aut NATIONALLICENCE 700 1- Huang Jiang Department of Parasitology, Guiyang Medical College, Guiyang, China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/3(2011-09-01), 567-574 0932-0113 109:3<567 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer