caa a22 4500 445340134 CHVBK 20180317142809.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00436-011-2296-5 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2296-5 Comparative evaluation of immunization with recombinant protein and plasmid DNA vaccines of fusion antigen ROP2 and SAG1 from Toxoplasma gondii in mice: cellular and humoral immune responses [Elektronische Daten] [Wen-Shu Li, Qing-Xin Chen, Ju-Xiu Ye, Zi-Xin Xie, Jun Chen, Li-Fang Zhang] The aim of this work was to evaluate immune responses in BALB/c mice vaccinated subcutaneously by recombinant protein, or intramuscularly by plasmid DNA with fusion antigen of rhoptry protein 2 (ROP2) and major surface protein 1 (SAG1) from Toxoplasma gondii (T. gondii). BALB/c mice were immunized with one of three different antigen formulations respectively, which were rROP2-SAG1, pcROP2-SAG1, and pcROP2-SAG1 boosted with rROP2-SAG1. The production of IgG, IgG subclasses, lymphoproliferation, and level of gamma interferon (IFN-γ) were detected after vaccination. The animals vaccinated with rROP2-SAG1 quickly developed specific anti-TLA (T. gondii lysate antigen) antibodies, which continued to rise after immunization. However, production of IgG against TLA in mice vaccinated with pcROP2-SAG1 was relatively slow and maintained a high level after reaching plateau. There are more vigorous specific lymphoproliferative responses observed in mice of group rROP2-SAG1 than in pcROP2-SAG1. Immune responses in mice of group pcROP2-SAG1 boosted with rROP2-SAG1 were similar to the protein immunization group. Three immunization procedures resulted in a similar level of IFN-γ production. Our results indicate that BALB/c mice vaccinated by three immunization procedures induce similar humoral and cellular immunity against infection of T. gondii. Mice immunized with recombinant protein rROP2-SAG1 produce more humoral immune responses than mice immunized with other antigen formulations. Springer-Verlag, 2011 Li Wen-Shu Department of Microbiology and Immunology, Wenzhou Medical College, 325035,, Wenzhou, Zhejiang Province, China aut Chen Qing-Xin Department of Microbiology and Immunology, Wenzhou Medical College, 325035,, Wenzhou, Zhejiang Province, China aut Ye Ju-Xiu School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical College, Xueyuan Road, Wenzhou, Zhejiang, China aut Xie Zi-Xin Department of Microbiology and Immunology, Wenzhou Medical College, 325035,, Wenzhou, Zhejiang Province, China aut Chen Jun Department of Microbiology and Immunology, Wenzhou Medical College, 325035,, Wenzhou, Zhejiang Province, China aut Zhang Li-Fang Department of Microbiology and Immunology, Wenzhou Medical College, 325035,, Wenzhou, Zhejiang Province, China aut Parasitology Research Springer-Verlag 109/3(2011-09-01), 637-644 0932-0113 109:3<637 2011 109 436 https://doi.org/10.1007/s00436-011-2296-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2296-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Wen-Shu Department of Microbiology and Immunology, Wenzhou Medical College, 325035,, Wenzhou, Zhejiang Province, China aut NATIONALLICENCE 700 1- Chen Qing-Xin Department of Microbiology and Immunology, Wenzhou Medical College, 325035,, Wenzhou, Zhejiang Province, China aut NATIONALLICENCE 700 1- Ye Ju-Xiu School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical College, Xueyuan Road, Wenzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Xie Zi-Xin Department of Microbiology and Immunology, Wenzhou Medical College, 325035,, Wenzhou, Zhejiang Province, China aut NATIONALLICENCE 700 1- Chen Jun Department of Microbiology and Immunology, Wenzhou Medical College, 325035,, Wenzhou, Zhejiang Province, China aut NATIONALLICENCE 700 1- Zhang Li-Fang Department of Microbiology and Immunology, Wenzhou Medical College, 325035,, Wenzhou, Zhejiang Province, China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/3(2011-09-01), 637-644 0932-0113 109:3<637 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer