caa a22 4500 445340177 CHVBK 20180317142809.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00436-011-2298-3 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2298-3 Candidate genes involving in tumorigenesis of cholangiocarcinoma induced by Opisthorchis viverrini infection [Elektronische Daten] [Zhiliang Wu, Thidarut Boonmars, Sirintip Boonjaraspinyo, Isao Nagano, Somchai Pinlaor, Anucha Puapairoj, Puangrat Yongvanit, Yuzo Takahashi] Opisthorchiasis-associated cholangiocarcinoma (CCA) is one of main public health problems in Opisthorchis viverrini endemic areas. Although the definite relationship between prevalence of CCA and the parasite infection has been demonstrated, the molecular mechanism of tumorigenesis is still unknown. In the present study, by using animal model of opisthorchiasis-associated CCA, a kinetic analysis of cDNA microarray was performed to screen the candidate genes that involve in the development of opisthorchiasis-associated CCA. Microarray analysis revealed that the expressions of 131 genes were up-regulated during the development of CCA, including the genes relative to cell proliferation, differentiation and transformation, cell growth and cycle regulation, apoptosis, DNA repair, and cytoskeletal structure. The expressions of 145 genes were down-regulated, including the genes relative to metabolic enzymes, tumor suppressor, apoptosis, and oxidative response and oxidation reduction. The present study listed up the candidate genes involving tumorigenesis, provided molecular information on the development of opisthorchiasis-associated CCA and the potential biomarkers for diagnosis and therapy, and suggested that the increased expression of cell differentiation, proliferation, transformation-related genes, and decreased expression of metabolic enzymes may play important roles in the tumorigenesis of CCA. Springer-Verlag, 2011 Wu Zhiliang Department of Parasitology, Gifu University Graduate School of Medicine, Yanagido1-1, 501-1194, Gifu, Japan aut Boonmars Thidarut Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut Boonjaraspinyo Sirintip Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut Nagano Isao Department of Parasitology, Gifu University Graduate School of Medicine, Yanagido1-1, 501-1194, Gifu, Japan aut Pinlaor Somchai Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut Puapairoj Anucha Department of Pathology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut Yongvanit Puangrat Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut Takahashi Yuzo Department of Parasitology, Gifu University Graduate School of Medicine, Yanagido1-1, 501-1194, Gifu, Japan aut Parasitology Research Springer-Verlag 109/3(2011-09-01), 657-673 0932-0113 109:3<657 2011 109 436 https://doi.org/10.1007/s00436-011-2298-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2298-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wu Zhiliang Department of Parasitology, Gifu University Graduate School of Medicine, Yanagido1-1, 501-1194, Gifu, Japan aut NATIONALLICENCE 700 1- Boonmars Thidarut Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Boonjaraspinyo Sirintip Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Nagano Isao Department of Parasitology, Gifu University Graduate School of Medicine, Yanagido1-1, 501-1194, Gifu, Japan aut NATIONALLICENCE 700 1- Pinlaor Somchai Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Puapairoj Anucha Department of Pathology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Yongvanit Puangrat Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Takahashi Yuzo Department of Parasitology, Gifu University Graduate School of Medicine, Yanagido1-1, 501-1194, Gifu, Japan aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/3(2011-09-01), 657-673 0932-0113 109:3<657 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer