caa a22 4500 445340193 CHVBK 20180317142810.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00436-011-2336-1 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2336-1 Molecular characterization of Cryptosporidium spp. in dairy calves from the state of São Paulo, Brazil [Elektronische Daten] [Marcelo Meireles, Fernando de Oliveira, Weslen Teixeira, William Coelho, Luiz Mendes] Cryptosporidiosis is a common protozoan disease observed in a wide range of vertebrate hosts, including ruminants. Cattle can be a potential reservoir of Cryptosporidium spp., leading to environmental contamination with oocysts of zoonotic species. The molecular characterization of Cryptosporidium spp. isolated from cattle from the state of São Paulo, Brazil, was accomplished using nested polymerase chain reaction for amplification of fragments of the 18S rRNA gene and the glycoprotein GP60 gene, following sequencing of amplified fragments. Positivity for Cryptosporidium was found in 10.7% (21/196) of the samples. Four species of Cryptosporidium were identified: C. andersoni, C. bovis, C. parvum subtype IIaA15G2R1, and C. ryanae. To the best of our knowledge, this is the first report of infection by C. ryanae and C. parvum IIaA15G2R1 in cattle from Brazil. Springer-Verlag, 2011 Meireles Marcelo Faculdade de Medicina Veterinária—Univ Estadual Paulista—UNESP, Araçatuba, Brazil aut de Oliveira Fernando Universidade Estadual Paulista—UNESP, São Paulo, Brazil aut Teixeira Weslen Universidade Estadual Paulista—UNESP, São Paulo, Brazil aut Coelho William Universidade Estadual Paulista—UNESP, São Paulo, Brazil aut Mendes Luiz Faculdade de Medicina Veterinária—Univ Estadual Paulista—UNESP, Araçatuba, Brazil aut Parasitology Research Springer-Verlag 109/3(2011-09-01), 949-951 0932-0113 109:3<949 2011 109 436 https://doi.org/10.1007/s00436-011-2336-1 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2336-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Meireles Marcelo Faculdade de Medicina Veterinária—Univ Estadual Paulista—UNESP, Araçatuba, Brazil aut NATIONALLICENCE 700 1- de Oliveira Fernando Universidade Estadual Paulista—UNESP, São Paulo, Brazil aut NATIONALLICENCE 700 1- Teixeira Weslen Universidade Estadual Paulista—UNESP, São Paulo, Brazil aut NATIONALLICENCE 700 1- Coelho William Universidade Estadual Paulista—UNESP, São Paulo, Brazil aut NATIONALLICENCE 700 1- Mendes Luiz Faculdade de Medicina Veterinária—Univ Estadual Paulista—UNESP, Araçatuba, Brazil aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/3(2011-09-01), 949-951 0932-0113 109:3<949 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer