caa a22 4500 445340215 CHVBK 20180317142810.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00436-011-2303-x doi (NATIONALLICENCE)springer-10.1007/s00436-011-2303-x Molecular cloning, expression, and immunolocalization of the NAD+-dependent glycerol 3-phosphate dehydrogenase (GPD) from Clonorchis sinensis [Elektronische Daten] [Yongxiu Fan, Xiaoyun Wang, Chuanhuan Deng, Yan Huang, Lexun Wang, Wenjun Chen, Chi Liang, Xuerong Li, Zhongdao Wu, Xinbing Yu] Glycerol 3-phosphate dehydrogenase (GPD) plays an important role in the energy metabolism and nutrition metabolism. In order to know about the biological functions of GPD of Clonorchis sinensis (C. sinensis), we identified a complete gene coding GPD from C. sinensis metacercaria cDNA library. This novel cDNA sequence contains 1,056bp with a putative open reading frame of 351 amino acids and shares 74% identity with GPD from Schistosoma mansoni. Recombinant CsGPD was expressed and purified from Escherichia coli BL21 (DE3). Western blot analysis displayed that recombinant CsGPD can be recognized by anti-CsGPD serum and C. sinensis-infected serum. RT-PCR and immunolocalization analysis confirmed that GPD expressed both at the stage of adult worm and metacercaria of C. sinensis and immunolocated at the tegument of adult worm, tegument and tegumentary cells of metacercaria. Our current study has paved the way for the further researches about the biological functions involved in the growth of C. sinensis. Springer-Verlag, 2011 Fan Yongxiu Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut Wang Xiaoyun Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut Deng Chuanhuan Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut Huang Yan Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut Wang Lexun Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut Chen Wenjun Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut Liang Chi Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut Li Xuerong Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut Wu Zhongdao Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut Yu Xinbing Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut Parasitology Research Springer-Verlag 109/3(2011-09-01), 621-626 0932-0113 109:3<621 2011 109 436 https://doi.org/10.1007/s00436-011-2303-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2303-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fan Yongxiu Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Wang Xiaoyun Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Deng Chuanhuan Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Huang Yan Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Wang Lexun Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Chen Wenjun Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Liang Chi Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Li Xuerong Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Wu Zhongdao Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Yu Xinbing Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/3(2011-09-01), 621-626 0932-0113 109:3<621 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer