caa a22 4500 445340231 CHVBK 20180317142810.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00436-011-2318-3 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2318-3 Survival of anopheline eggs and their susceptibility to infection with Metarhizium anisopliae and Beauveria bassiana under laboratory conditions [Elektronische Daten] [Christian Luz, Ladslaus Mnyone, Tanya Russell] The viability of Anopheles gambiae sensu stricto and Anopheles arabiensis (Diptera: Culicidae) eggs over time and the ovicidal activity of Beauveria bassiana (Ascomycota: Cordycipitaceae) and Metarhizium anisopliae (Ascomycota: Clavicipitaceae) were investigated. Eggs were incubated in soil or leaf litter for up to 12weeks at 26°C and 75%, 86% or >98% relative humidity (RH). Eggs were treated topically with M. anisopliae ICIPE-30 or B. bassiana I93-825 conidia in either water or oil-in-water formulations. Survival of eggs whether treated or not with fungus was similar, and untreated eggs generally did not survive longer than 2weeks regardless of the substrate or humidity tested. After a minimal 5-day exposure, M. anisopliae at 5 × 106conidia/cm2 clearly reduced the number of larvae. The efficacy of the fungus increased when it was oil-in-water formulated, and eclosion was completely prevented regardless of the conidial concentration (105-107conidia/cm2) after a 10-day exposure in soils at >98% RH. Treatment of eggs with B. bassiana, however, failed to reduce the number of eclosing larvae. This is the first demonstration of the ovicidal activity by M. anisopliae against either A. gambiae s. s. or A. arabiensis and the results underline the potential of this fungus against anopheline mosquitoes. Springer-Verlag, 2011 Luz Christian Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, CP 131, 74001-970, Goiânia, GO, Brazil aut Mnyone Ladslaus Biomedical and Environmental Group, Ifakara Health Institute, Off Mlabani Passage, P.O. Box53, Ifakara, Tanzania aut Russell Tanya Biomedical and Environmental Group, Ifakara Health Institute, Off Mlabani Passage, P.O. Box53, Ifakara, Tanzania aut Parasitology Research Springer-Verlag 109/3(2011-09-01), 751-758 0932-0113 109:3<751 2011 109 436 https://doi.org/10.1007/s00436-011-2318-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2318-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Luz Christian Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, CP 131, 74001-970, Goiânia, GO, Brazil aut NATIONALLICENCE 700 1- Mnyone Ladslaus Biomedical and Environmental Group, Ifakara Health Institute, Off Mlabani Passage, P.O. Box53, Ifakara, Tanzania aut NATIONALLICENCE 700 1- Russell Tanya Biomedical and Environmental Group, Ifakara Health Institute, Off Mlabani Passage, P.O. Box53, Ifakara, Tanzania aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/3(2011-09-01), 751-758 0932-0113 109:3<751 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer