caa a22 4500 445340290 CHVBK 20180317142810.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00436-011-2305-8 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2305-8 Neutrophil and eosinophil chemotactic factors in the excretory/secretory products of sheep abomasal nematode parasites [Elektronische Daten] NCF and ECF in abomasal nematodes [Stefanie Reinhardt, Ian Scott, Heather Simpson] Both eosinophil chemotactic factor (ECF) and neutrophil chemotactic factor (NCF) activities were demonstrated in excretory/secretory (ES) products and homogenates of Haemonchus contortus and Teladorsagia circumcincta larvae and adult worms in a modified checkerboard assay using a micro-chemotaxis chamber. Neutrophil chemotaxis was seen in 28 of 35 experiments and eosinophil chemotaxis in 20 of 38 experiments. Chemokinetic activity for neutrophils and eosinophils (accounting for 40-50% of total cell migration) was also apparent in only three parasite products for each cell type. Significant NCF activity was present in six of seven adult worm ES products (three of four from T. circumcincta and in all three from H. contortus) and ECF activity in four of five adult ES products, whereas fewer L3 incubates, particularly of T. circumcincta, contained chemotactic activity. All parasite homogenates, with one exception for ECF, were chemotactic for both neutrophils and eosinophils. The sequential use of cellulose ultrafiltration membranes of decreasing pore size did not identify precisely the molecular weight of the NCF and ECF but indicated that the active chemicals were greater than 10kDa and probably greater than 30kDa. Springer-Verlag, 2011 Reinhardt Stefanie S. Reinhardt and B.Ullrich, Tierärztliche Gemeinschaftspraxis, Im Stocken 8A, 21255, Tostedt, Germany aut Scott Ian Institute of Veterinary, Animal and Biomedical Sciences, Massey University, 4442, Palmerston North, New Zealand aut Simpson Heather Institute of Veterinary, Animal and Biomedical Sciences, Massey University, 4442, Palmerston North, New Zealand aut Parasitology Research Springer-Verlag 109/3(2011-09-01), 627-635 0932-0113 109:3<627 2011 109 436 https://doi.org/10.1007/s00436-011-2305-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2305-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Reinhardt Stefanie S. Reinhardt and B.Ullrich, Tierärztliche Gemeinschaftspraxis, Im Stocken 8A, 21255, Tostedt, Germany aut NATIONALLICENCE 700 1- Scott Ian Institute of Veterinary, Animal and Biomedical Sciences, Massey University, 4442, Palmerston North, New Zealand aut NATIONALLICENCE 700 1- Simpson Heather Institute of Veterinary, Animal and Biomedical Sciences, Massey University, 4442, Palmerston North, New Zealand aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/3(2011-09-01), 627-635 0932-0113 109:3<627 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer