caa a22 4500 445340320 CHVBK 20180317142810.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00436-011-2294-7 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2294-7 Molecular subtyping of Blastocystis sp. isolates from symptomatic patients in Italy [Elektronische Daten] [Dionigia Meloni, Giovanna Sanciu, Philippe Poirier, Hicham El Alaoui, Magali Chabé, Laurence Delhaes, Eduardo Dei-Cas, Frederic Delbac, Pier Luigi Fiori, David Di Cave, Eric Viscogliosi] Blastocystis sp. is the most common eukaryotic parasite in the intestinal tract of humans. Due to its potential impact in public health, we determined the Blastocystis sp. subtypes (STs) and their relative frequency in symptomatic patients living in or in the vicinity of two Italian cities (Rome and Sassari). A total of 34 Blastocystis sp. isolates corresponding to 26 single and 4 mixed infections were subtyped using partial small subunit ribosomal RNA gene sequencing. From this molecular approach, the ST distribution in the present Italian population was as follows: ST3 (47.1%), ST2 (20.6%), ST4 (17.7%), ST1 (8.8%), and ST7, and ST8 (2.9%). As in almost all countries worldwide, ST3 was the most common ST reinforcing the hypothesis of its human origin. Together with a previous preliminary report, a total of seven STs (with the addition of ST5) have been found in Italian symptomatic patients. The wide range of STs identified in the Italian population suggest that Blastocystis sp. infection is not associated with specific STs even if some STs (ST1-ST4) are predominant as reported in all other countries. Since most of the STs identified in Italian patients are zoonotic, our data raise crucial questions concerning the identification of animal reservoirs for Blastocystis sp. and the potential risks of transmission to humans. Springer-Verlag, 2011 Meloni Dionigia Center for Infection and Immunity of Lille (CIIL), Institut Pasteur of Lille, Inserm U1019, CNRS UMR 8204, University Lille Nord de France, Biology and Diversity of Emerging Eukaryotic Pathogens (BDEEP), 1 rue du Professeur Calmette, BP 245, 59019, Lille Cedex, France aut Sanciu Giovanna Center for Infection and Immunity of Lille (CIIL), Institut Pasteur of Lille, Inserm U1019, CNRS UMR 8204, University Lille Nord de France, Biology and Diversity of Emerging Eukaryotic Pathogens (BDEEP), 1 rue du Professeur Calmette, BP 245, 59019, Lille Cedex, France aut Poirier Philippe Laboratoire Microorganismes: Génome et Environnement (LMGE), CNRS UMR 6023, University Blaise Pascal of Clermont-Ferrand, Avenue des Landais, 63177, Aubière Cedex, France aut El Alaoui Hicham Laboratoire Microorganismes: Génome et Environnement (LMGE), CNRS UMR 6023, University Blaise Pascal of Clermont-Ferrand, Avenue des Landais, 63177, Aubière Cedex, France aut Chabé Magali Center for Infection and Immunity of Lille (CIIL), Institut Pasteur of Lille, Inserm U1019, CNRS UMR 8204, University Lille Nord de France, Biology and Diversity of Emerging Eukaryotic Pathogens (BDEEP), 1 rue du Professeur Calmette, BP 245, 59019, Lille Cedex, France aut Delhaes Laurence Center for Infection and Immunity of Lille (CIIL), Institut Pasteur of Lille, Inserm U1019, CNRS UMR 8204, University Lille Nord de France, Biology and Diversity of Emerging Eukaryotic Pathogens (BDEEP), 1 rue du Professeur Calmette, BP 245, 59019, Lille Cedex, France aut Dei-Cas Eduardo Center for Infection and Immunity of Lille (CIIL), Institut Pasteur of Lille, Inserm U1019, CNRS UMR 8204, University Lille Nord de France, Biology and Diversity of Emerging Eukaryotic Pathogens (BDEEP), 1 rue du Professeur Calmette, BP 245, 59019, Lille Cedex, France aut Delbac Frederic Laboratoire Microorganismes: Génome et Environnement (LMGE), CNRS UMR 6023, University Blaise Pascal of Clermont-Ferrand, Avenue des Landais, 63177, Aubière Cedex, France aut Luigi Fiori Pier Department of Biomedical Sciences, Division of Experimental and Clinical Microbiology, University of Sassari, Viale San Pietro 43/B, 07100, Sassari, Italy aut Di Cave David Department of Public Health and Cell Biology, University of Rome Tor Vergata, Via Montpellier 1, 00133, Rome, Italy aut Viscogliosi Eric Center for Infection and Immunity of Lille (CIIL), Institut Pasteur of Lille, Inserm U1019, CNRS UMR 8204, University Lille Nord de France, Biology and Diversity of Emerging Eukaryotic Pathogens (BDEEP), 1 rue du Professeur Calmette, BP 245, 59019, Lille Cedex, France aut Parasitology Research Springer-Verlag 109/3(2011-09-01), 613-619 0932-0113 109:3<613 2011 109 436 https://doi.org/10.1007/s00436-011-2294-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2294-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Meloni Dionigia Center for Infection and Immunity of Lille (CIIL), Institut Pasteur of Lille, Inserm U1019, CNRS UMR 8204, University Lille Nord de France, Biology and Diversity of Emerging Eukaryotic Pathogens (BDEEP), 1 rue du Professeur Calmette, BP 245, 59019, Lille Cedex, France aut NATIONALLICENCE 700 1- Sanciu Giovanna Center for Infection and Immunity of Lille (CIIL), Institut Pasteur of Lille, Inserm U1019, CNRS UMR 8204, University Lille Nord de France, Biology and Diversity of Emerging Eukaryotic Pathogens (BDEEP), 1 rue du Professeur Calmette, BP 245, 59019, Lille Cedex, France aut NATIONALLICENCE 700 1- Poirier Philippe Laboratoire Microorganismes: Génome et Environnement (LMGE), CNRS UMR 6023, University Blaise Pascal of Clermont-Ferrand, Avenue des Landais, 63177, Aubière Cedex, France aut NATIONALLICENCE 700 1- El Alaoui Hicham Laboratoire Microorganismes: Génome et Environnement (LMGE), CNRS UMR 6023, University Blaise Pascal of Clermont-Ferrand, Avenue des Landais, 63177, Aubière Cedex, France aut NATIONALLICENCE 700 1- Chabé Magali Center for Infection and Immunity of Lille (CIIL), Institut Pasteur of Lille, Inserm U1019, CNRS UMR 8204, University Lille Nord de France, Biology and Diversity of Emerging Eukaryotic Pathogens (BDEEP), 1 rue du Professeur Calmette, BP 245, 59019, Lille Cedex, France aut NATIONALLICENCE 700 1- Delhaes Laurence Center for Infection and Immunity of Lille (CIIL), Institut Pasteur of Lille, Inserm U1019, CNRS UMR 8204, University Lille Nord de France, Biology and Diversity of Emerging Eukaryotic Pathogens (BDEEP), 1 rue du Professeur Calmette, BP 245, 59019, Lille Cedex, France aut NATIONALLICENCE 700 1- Dei-Cas Eduardo Center for Infection and Immunity of Lille (CIIL), Institut Pasteur of Lille, Inserm U1019, CNRS UMR 8204, University Lille Nord de France, Biology and Diversity of Emerging Eukaryotic Pathogens (BDEEP), 1 rue du Professeur Calmette, BP 245, 59019, Lille Cedex, France aut NATIONALLICENCE 700 1- Delbac Frederic Laboratoire Microorganismes: Génome et Environnement (LMGE), CNRS UMR 6023, University Blaise Pascal of Clermont-Ferrand, Avenue des Landais, 63177, Aubière Cedex, France aut NATIONALLICENCE 700 1- Luigi Fiori Pier Department of Biomedical Sciences, Division of Experimental and Clinical Microbiology, University of Sassari, Viale San Pietro 43/B, 07100, Sassari, Italy aut NATIONALLICENCE 700 1- Di Cave David Department of Public Health and Cell Biology, University of Rome Tor Vergata, Via Montpellier 1, 00133, Rome, Italy aut NATIONALLICENCE 700 1- Viscogliosi Eric Center for Infection and Immunity of Lille (CIIL), Institut Pasteur of Lille, Inserm U1019, CNRS UMR 8204, University Lille Nord de France, Biology and Diversity of Emerging Eukaryotic Pathogens (BDEEP), 1 rue du Professeur Calmette, BP 245, 59019, Lille Cedex, France aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/3(2011-09-01), 613-619 0932-0113 109:3<613 2011 109 436 Metadata rights reserved Springer special 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