caa a22 4500 445340355 CHVBK 20180317142810.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00436-011-2323-6 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2323-6 Genetic analysis of Fasciola isolates from cattle in Korea based on second internal transcribed spacer (ITS-2) sequence of nuclear ribosomal DNA [Elektronische Daten] [Se-Eun Choe, Thuy Nguyen, Tae-Gyu Kang, Chang-Hee Kweon, Seung-Won Kang] Nuclear ribosomal DNA sequence of the second internal transcribed spacer (ITS-2) has been used efficiently to identify the liver fluke species collected from different hosts and various geographic regions. ITS-2 sequences of 19 Fasciola samples collected from Korean native cattle were determined and compared. Sequence comparison including ITS-2 sequences of isolates from this study and reference sequences from Fasciola hepatica and Fasciola gigantica and intermediate Fasciola in Genbank revealed seven identical variable sites of investigated isolates. Among 19 samples, 12 individuals had ITS-2 sequences completely identical to that of pure F. hepatica, five possessed the sequences identical to F. gigantica type, whereas two shared the sequence of both F. hepatica and F. gigantica. No variations in length and nucleotide composition of ITS-2 sequence were observed within isolates that belonged to F. hepatica or F. gigantica. At the position of 218, five Fasciola containing a single-base substitution (C>T) formed a distinct branch inside the F. gigantica-type group which was similar to those of Asian-origin isolates. The phylogenetic tree of the Fasciola spp. based on complete ITS-2 sequences from this study and other representative isolates in different locations clearly showed that pure F. hepatica, F. gigantica type and intermediate Fasciola were observed. The result also provided additional genetic evidence for the existence of three forms of Fasciola isolated from native cattle in Korea by genetic approach using ITS-2 sequence. Springer-Verlag, 2011 Choe Se-Eun National Veterinary Research and Quarantine Service (NVRQS), 480 Anyang6 dong, 420-480, Anyang City, South Korea aut Nguyen Thuy National Veterinary Research and Quarantine Service (NVRQS), 480 Anyang6 dong, 420-480, Anyang City, South Korea aut Kang Tae-Gyu Nothern Branch Veterinary Service Lab, 217-130, Sokcho City, Gangwon Province, South Korea aut Kweon Chang-Hee National Veterinary Research and Quarantine Service (NVRQS), 480 Anyang6 dong, 420-480, Anyang City, South Korea aut Kang Seung-Won National Veterinary Research and Quarantine Service (NVRQS), 480 Anyang6 dong, 420-480, Anyang City, South Korea aut Parasitology Research Springer-Verlag 109/3(2011-09-01), 833-839 0932-0113 109:3<833 2011 109 436 https://doi.org/10.1007/s00436-011-2323-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2323-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Choe Se-Eun National Veterinary Research and Quarantine Service (NVRQS), 480 Anyang6 dong, 420-480, Anyang City, South Korea aut NATIONALLICENCE 700 1- Nguyen Thuy National Veterinary Research and Quarantine Service (NVRQS), 480 Anyang6 dong, 420-480, Anyang City, South Korea aut NATIONALLICENCE 700 1- Kang Tae-Gyu Nothern Branch Veterinary Service Lab, 217-130, Sokcho City, Gangwon Province, South Korea aut NATIONALLICENCE 700 1- Kweon Chang-Hee National Veterinary Research and Quarantine Service (NVRQS), 480 Anyang6 dong, 420-480, Anyang City, South Korea aut NATIONALLICENCE 700 1- Kang Seung-Won National Veterinary Research and Quarantine Service (NVRQS), 480 Anyang6 dong, 420-480, Anyang City, South Korea aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/3(2011-09-01), 833-839 0932-0113 109:3<833 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer