caa a22 4500 445340436 CHVBK 20180317142810.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00436-011-2322-7 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2322-7 Optimizing conditions for the use of chlorophyll derivatives for photodynamic control of parasites in aquatic ecosystems [Elektronische Daten] [Gilmar Erzinger, Stephanie Wohllebe, Fernanda Vollrath, Suellen Souza, Peter Richter, Michael Lebert, Donat-Peter Häder] Recently, it was demonstrated that mosquito larvae can be killed by means of photodynamic processes after the larvae have incorporated the photosensitizer chlorophyllin or pheophorbid, and were treated with light. The water-soluble substances were applied to and incorporated by the larvae in darkness. With Chaoborus sp. a dark incubation of about 3h is sufficient to yield mortality of about 90% and ≥6h resulted in almost 100% mortality during subsequent illumination. Temperature did not influence mortality of the larvae significantly in a treatment of 6h dark incubation and subsequent 3h illumination. At 10°C, 20°C, or 30°C, between 80% and 100% of the treated larvae died when the light intensity from a solar simulator was above 30W/m2. Lower irradiances were less effective. The LD50 value of magnesium chlorophyllin was about 22.25mg/l and for Zn chlorophyll 17.53mg/l, while Cu chlorophyll (LD50 0.1mg/l) was shown to be toxic also without light. Chlorophyllin, which was lyophilized immediately after extraction, was far more lethal to the larvae (LD50 14.88mg/l) than air-dried Mg chlorophyllin. Springer-Verlag, 2011 Erzinger Gilmar Department of Pharmacy and Medicine, University of Joinville Region, Rua Paulo Malschitzki, n° 10 Campus Universitário, Joinville, Brazil aut Wohllebe Stephanie Bavarian State Research Centre for Agriculture, Institute for Fisheries, Starnberg, Baden-Württemberg, Germany aut Vollrath Fernanda Department of Pharmacy and Medicine, University of Joinville Region, Rua Paulo Malschitzki, n° 10 Campus Universitário, Joinville, Brazil aut Souza Suellen Department of Pharmacy and Medicine, University of Joinville Region, Rua Paulo Malschitzki, n° 10 Campus Universitário, Joinville, Brazil aut Richter Peter Department of Biology, Cell Biology, Friedrich-Alexander-University Erlangen-Nuremberg, Staudtstrasse 5, 91058, Erlangen, Germany aut Lebert Michael Department of Biology, Cell Biology, Friedrich-Alexander-University Erlangen-Nuremberg, Staudtstrasse 5, 91058, Erlangen, Germany aut Häder Donat-Peter Neue Str. 9, Möhrendorf, Germany aut Parasitology Research Springer-Verlag 109/3(2011-09-01), 781-786 0932-0113 109:3<781 2011 109 436 https://doi.org/10.1007/s00436-011-2322-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2322-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Erzinger Gilmar Department of Pharmacy and Medicine, University of Joinville Region, Rua Paulo Malschitzki, n° 10 Campus Universitário, Joinville, Brazil aut NATIONALLICENCE 700 1- Wohllebe Stephanie Bavarian State Research Centre for Agriculture, Institute for Fisheries, Starnberg, Baden-Württemberg, Germany aut NATIONALLICENCE 700 1- Vollrath Fernanda Department of Pharmacy and Medicine, University of Joinville Region, Rua Paulo Malschitzki, n° 10 Campus Universitário, Joinville, Brazil aut NATIONALLICENCE 700 1- Souza Suellen Department of Pharmacy and Medicine, University of Joinville Region, Rua Paulo Malschitzki, n° 10 Campus Universitário, Joinville, Brazil aut NATIONALLICENCE 700 1- Richter Peter Department of Biology, Cell Biology, Friedrich-Alexander-University Erlangen-Nuremberg, Staudtstrasse 5, 91058, Erlangen, Germany aut NATIONALLICENCE 700 1- Lebert Michael Department of Biology, Cell Biology, Friedrich-Alexander-University Erlangen-Nuremberg, Staudtstrasse 5, 91058, Erlangen, Germany aut NATIONALLICENCE 700 1- Häder Donat-Peter Neue Str. 9, Möhrendorf, Germany aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/3(2011-09-01), 781-786 0932-0113 109:3<781 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer