caa a22 4500 445340479 CHVBK 20180317142810.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00436-011-2328-1 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2328-1 Larvicidal potential of silver nanoparticles synthesized using fungus Cochliobolus lunatus against Aedes aegypti (Linnaeus, 1762) and Anopheles stephensi Liston (Diptera; Culicidae) [Elektronische Daten] [Rahul Salunkhe, Satish Patil, Chandrashekhar Patil, Bipinchandra Salunke] Larvicides play a vital role in controlling mosquitoes in their breeding sites. The present study was carried out to establish the larvicidal activities of mycosynthesized silver nanoparticles (AgNPs) against vectors: Aedes aegypti and Anopheles stephensi responsible for diseases of public health importance. The AgNPs synthesized by filamentous fungus Cochliobolus lunatus, characterized by UV-Vis spectrophotometry, Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. The characterization studies confirmed the spherical shape and size (3-21nm) of silver nanoparticles. The efficacy of mycosynthesized AgNPs at all the tested concentrations (10, 5, 2.5, 1.25, 0.625, and 0.3125ppm) against second, third, and fourth instar larvae of A. aegypti (LC50 1.29, 1.48, and 1.58; LC90 3.08, 3.33, and 3.41ppm) and against A. stephensi (LC50 1.17, 1.30, and 1.41; LC90 2.99, 3.13, and 3.29ppm) were observed, respectively. The mortality rates were positively correlated with the concentration of AgNPs. Significant (P < 0.05) changes in the larval mortality was also recorded between the period of exposure against fourth instar larvae of A. aegypti and A. stephensi. The possible larvicidal activity may be due to penetration of nanoparticles through membrane. Toxicity studies carried out against non-target fish species Poecilia reticulata, the most common organism in the habitats of A. aegypti and A. stephensi showed no toxicity at LC50 and LC90 doses of the AgNPs. This is the first report on mosquito larvicidal activity of mycosynthesized nanoparticles. Thus, the use of fungus C. lunatus to synthesize silver nanoparticles is a rapid, eco-friendly, and a single-step approach and the AgNps formed can be potential mosquito larvicidal agents. Springer-Verlag, 2011 Salunkhe Rahul School of Life Sciences, North Maharashtra University, Post Box-80, 425001, Jalgaon, Maharashtra, India aut Patil Satish School of Life Sciences, North Maharashtra University, Post Box-80, 425001, Jalgaon, Maharashtra, India aut Patil Chandrashekhar School of Life Sciences, North Maharashtra University, Post Box-80, 425001, Jalgaon, Maharashtra, India aut Salunke Bipinchandra School of Life Sciences, North Maharashtra University, Post Box-80, 425001, Jalgaon, Maharashtra, India aut Parasitology Research Springer-Verlag 109/3(2011-09-01), 823-831 0932-0113 109:3<823 2011 109 436 https://doi.org/10.1007/s00436-011-2328-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2328-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Salunkhe Rahul School of Life Sciences, North Maharashtra University, Post Box-80, 425001, Jalgaon, Maharashtra, India aut NATIONALLICENCE 700 1- Patil Satish School of Life Sciences, North Maharashtra University, Post Box-80, 425001, Jalgaon, Maharashtra, India aut NATIONALLICENCE 700 1- Patil Chandrashekhar School of Life Sciences, North Maharashtra University, Post Box-80, 425001, Jalgaon, Maharashtra, India aut NATIONALLICENCE 700 1- Salunke Bipinchandra School of Life Sciences, North Maharashtra University, Post Box-80, 425001, Jalgaon, Maharashtra, India aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/3(2011-09-01), 823-831 0932-0113 109:3<823 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer