caa a22 4500 445340495 CHVBK 20180317142811.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00436-011-2288-5 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2288-5 Potentially pathogenic free-living amoebae isolated from hospital wards with immunodeficient patients in Tehran, Iran [Elektronische Daten] [Zohreh Lasjerdi, Maryam Niyyati, Ali Haghighi, Saed Shahabi, Farid Biderouni, Niloofar Taghipour, Mohamad Eftekhar, Ehsan Nazemalhosseini Mojarad] This study investigated the occurrence of free-living amoebae (FLA) in immunodeficiency wards of hospitals in Tehran, Iran. A total of 70 dust and biofilm samples from wards serving transplant, pediatric (malignancies), HIV, leukemia and oncology patients of five university hospitals were collected and examined for the presence of FLA using culturing and molecular approaches. Based on the morphology of the amoebae in plate cultures, primer sets were applied for molecular identification of Acanthamoeba, vahlkampfiid amoebae and Hartmannella. Out of 70 samples, 37 (52.9%) were positive for FLA. Acanthamoeba belonged to the T4 genotype was the most prevalent isolate. Presence of the T4 genotype on medical instruments, including an oxygen mask in an isolation room of an immunodeficiency pediatric ward, should be of concern for health authorities. Acanthamoeba T5 genotypes, Hartmannella vermiformis, and Vahlkampfia avara were also present. These results highlight a clear need for greater attention to improved disinfection, especially where susceptible patients, such as those who are immune-suppressed, are served. To our knowledge, this is the first report of these FLA in immunodeficiency wards in Iran, and also the first to identify Acanthamoeba T5, Hartmannella, and Vahlkampfia in moist habitats, such as biofilms, in this country. Springer-Verlag, 2011 Lasjerdi Zohreh Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut Niyyati Maryam Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut Haghighi Ali Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut Shahabi Saed Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut Biderouni Farid Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut Taghipour Niloofar Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut Eftekhar Mohamad Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut Nazemalhosseini Mojarad Ehsan Research Center for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut Parasitology Research Springer-Verlag 109/3(2011-09-01), 575-580 0932-0113 109:3<575 2011 109 436 https://doi.org/10.1007/s00436-011-2288-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2288-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lasjerdi Zohreh Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut NATIONALLICENCE 700 1- Niyyati Maryam Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut NATIONALLICENCE 700 1- Haghighi Ali Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut NATIONALLICENCE 700 1- Shahabi Saed Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut NATIONALLICENCE 700 1- Biderouni Farid Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut NATIONALLICENCE 700 1- Taghipour Niloofar Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut NATIONALLICENCE 700 1- Eftekhar Mohamad Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut NATIONALLICENCE 700 1- Nazemalhosseini Mojarad Ehsan Research Center for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/3(2011-09-01), 575-580 0932-0113 109:3<575 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer