caa a22 4500 445340517 CHVBK 20180317142811.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00436-011-2317-4 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2317-4 Differential diagnosis of Triaenophorus crassus and T. nodulosus experimental infection in Cyclops abyssorum praealpinus (Copepoda) from the Alpine Lake Grundlsee (Austria) using PCR-RFLP [Elektronische Daten] [Belgees Boufana, Uroš Žibrat, Robert Jehle, Philip Craig, Hubert Gassner, Robert Schabetsberger] In 2005, an epidemic of the cestode Triaenophorus crassus in Arctic charr, Salvelinus umbla from Lake Grundlsee (Austria) was reported, resulting in the closure of the local fisheries. The parasite uses a copepod and a salmonid fish as intermediate hosts, and the European pike (Esox lucius) as definitive host. In Lake Grundlsee procercoids of T. crassus, T. nodulosus and Eubothrium salvelini use the copepod Cyclops abyssorum praealpinus as their first intermediate host. We report on the development of a PCR-RFLP assay for the specific differentiation between the procercoids of the three cestode species. Ban I restriction sites within a 207-bp 18S rRNA-amplified fragment yielded two and three species-specific products to unequivocally distinguish between T. crassus and T. nodulosus, respectively. The assay is sufficiently sensitive to characterise a single procercoid of both Triaenophorus species in their copepod host, enabling a direct measure of infection. This assay will be useful in monitoring the progress of the control methods currently in place. Springer-Verlag, 2011 Boufana Belgees School of Environment and Life Sciences, University of Salford, Salford Crescent, M5 4WT, Salford, Greater Manchester, UK aut Žibrat Uroš Department of Freshwater and Terrestrial Ecosystems Research, National Institute of Biology, Večna pot 111, 1000, Ljubljana, Slovenia aut Jehle Robert School of Environment and Life Sciences, University of Salford, Salford Crescent, M5 4WT, Salford, Greater Manchester, UK aut Craig Philip School of Environment and Life Sciences, University of Salford, Salford Crescent, M5 4WT, Salford, Greater Manchester, UK aut Gassner Hubert Federal Agency for Water Management, Institute of Water Ecology, Fisheries and Lake Research, Scharfling 18, 5310, Mondsee, Austria aut Schabetsberger Robert Department of Organismic Biology, University of Salzburg, Hellbrunnerstrasse 34, 5020, Salzburg, Austria aut Parasitology Research Springer-Verlag 109/3(2011-09-01), 745-750 0932-0113 109:3<745 2011 109 436 https://doi.org/10.1007/s00436-011-2317-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2317-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Boufana Belgees School of Environment and Life Sciences, University of Salford, Salford Crescent, M5 4WT, Salford, Greater Manchester, UK aut NATIONALLICENCE 700 1- Žibrat Uroš Department of Freshwater and Terrestrial Ecosystems Research, National Institute of Biology, Večna pot 111, 1000, Ljubljana, Slovenia aut NATIONALLICENCE 700 1- Jehle Robert School of Environment and Life Sciences, University of Salford, Salford Crescent, M5 4WT, Salford, Greater Manchester, UK aut NATIONALLICENCE 700 1- Craig Philip School of Environment and Life Sciences, University of Salford, Salford Crescent, M5 4WT, Salford, Greater Manchester, UK aut NATIONALLICENCE 700 1- Gassner Hubert Federal Agency for Water Management, Institute of Water Ecology, Fisheries and Lake Research, Scharfling 18, 5310, Mondsee, Austria aut NATIONALLICENCE 700 1- Schabetsberger Robert Department of Organismic Biology, University of Salzburg, Hellbrunnerstrasse 34, 5020, Salzburg, Austria aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/3(2011-09-01), 745-750 0932-0113 109:3<745 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer