caa a22 4500 445340525 CHVBK 20180317142811.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00436-011-2316-5 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2316-5 Proteomic analysis of excretory secretory products from Clonorchis sinensis adult worms: molecular characterization and serological reactivity of a excretory-secretory antigen-fructose-1,6-bisphosphatase [Elektronische Daten] [Minghui Zheng, Kunhua Hu, Wei Liu, Xuchu Hu, Fengyu Hu, Lisi Huang, Peng Wang, Yue Hu, Yan Huang, Wenfang Li, Chi Liang, Xingfeng Yin, Qingyu He, Xinbing Yu] Clonorchis sinensis is a food-borne zoonotic parasite that resides in bile ducts and causes clonorchiasis, which may result in cholelithiasis, cholecystitis, hepatic fibrosis, and liver tumors. Although total excretory secretory products (ESP) of C. sinensis adults induce hepatic fibrosis in vivo in rats, the causative mechanism is not well understood. To study components of the ESP, C. sinensis culture medium was collected and analyzed using shotgun LC-MS/MS. We identified a total of 110 proteins, including glycometabolic enzymes (such as fructose-1,6-bisphosphatase (FBPase) and enolase), detoxification enzymes (such as glutamate dehydrogenase, dihydrolipoamide dehydrogenase and cathepsin B endopeptidase), and a number of RAB family proteins. To identify a potential causative agent for hepatic fibrosis, we expressed and purified a recombinant FBPase, a 1,041-bp gene product that encodes a 41.7-kDa protein with prototypical FBPase domains and that can form a tetramer with a molecular mass of 166.8kDa. In addition, we found that FBPase is an antigen present in the ESP and in circulation. Immunofluorescence showed that FBPase localizes to the intestinal cecum and vitellarium in C. sinensis adults. Our results describe the components of the excretory secretory products from C. sinensis adult worms and suggest that FBPase may be an important antigen present in the ESP of C. sinensis and may lay the foundation for additional studies on the development of clonorchiasis-associated hepatic fibrosis. Springer-Verlag, 2011 Zheng Minghui Department of Parasitology, Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut Hu Kunhua Proteomics Center, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut Liu Wei Guangdong Provincial Key Laboratory of Liver Disease, The Third Affiliated Hospital, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut Hu Xuchu Department of Parasitology, Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut Hu Fengyu Department of Parasitology, Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut Huang Lisi Department of Parasitology, Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut Wang Peng Department of Parasitology, Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut Hu Yue Department of Parasitology, Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut Huang Yan Department of Parasitology, Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut Li Wenfang Department of Parasitology, Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut Liang Chi Department of Parasitology, Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut Yin Xingfeng Institute of Life and Health Engineering, and National Engineering and Research Center for Genetic Medicine, Jinan University, 510632, Guangzhou, People's Republic of China aut He Qingyu Institute of Life and Health Engineering, and National Engineering and Research Center for Genetic Medicine, Jinan University, 510632, Guangzhou, People's Republic of China aut Yu Xinbing Department of Parasitology, Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut Parasitology Research Springer-Verlag 109/3(2011-09-01), 737-744 0932-0113 109:3<737 2011 109 436 https://doi.org/10.1007/s00436-011-2316-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2316-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zheng Minghui Department of Parasitology, Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Hu Kunhua Proteomics Center, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Liu Wei Guangdong Provincial Key Laboratory of Liver Disease, The Third Affiliated Hospital, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Hu Xuchu Department of Parasitology, Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Hu Fengyu Department of Parasitology, Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Huang Lisi Department of Parasitology, Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Wang Peng Department of Parasitology, Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Hu Yue Department of Parasitology, Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Huang Yan Department of Parasitology, Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Li Wenfang Department of Parasitology, Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Liang Chi Department of Parasitology, Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Yin Xingfeng Institute of Life and Health Engineering, and National Engineering and Research Center for Genetic Medicine, Jinan University, 510632, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- He Qingyu Institute of Life and Health Engineering, and National Engineering and Research Center for Genetic Medicine, Jinan University, 510632, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Yu Xinbing Department of Parasitology, Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/3(2011-09-01), 737-744 0932-0113 109:3<737 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer