caa a22 4500 445340568 CHVBK 20180317142811.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00436-011-2408-2 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2408-2 Efficacy of Toltrazuril 5 % Suspension against Eimeria bovis and Eimeria zuernii in Calves and Observations on the Associated Immunopathology [Elektronische Daten] [Nicholas Jonsson, Emily Piper, Christian Gray, Abdulkerim Deniz, Constantin Constantinoiu] 16 calves were each infected with suspensions containing a mixture of approximately 230,000 Eimeria bovis and 70,000 E. zuernii oocysts, which resulted in detection of oocysts in faeces of 12 of 16 calves by day +14 after infection. On day +14 after infection calves were either treated (n = 8) with toltrazuril at 15mg/kg body weight or with a placebo. Observations were made on the clinical condition, faecal score and liveweight of calves daily from one day post infection (pi) until 24days pi when all calves were euthanised and examined post mortem. Samples were collected from ileum and colon for histological, immunohistochemical and gene expression studies. The study demonstrated an efficacy of toltrazuril for the treatment of E. bovis and E. zuernii infections in calves reaching 99 % (based on arithmetic mean oocyst counts in faeces) within three days of treatment and remaining at or above this level for six days. Toltrazuril did not have a significant effect on the pattern and extent of immune cellular infiltration in the mucosa of ileum and colon, but the expression of the genes coding IL-2, IL-10 and TNF-α in the ileum and TNF-α in the colon were elevated in calves treated with toltrazuril. Higher levels of oocyst shedding were significantly associated with lower expression of genes coding for IL-2, IL-10 and higher IP-10. It is concluded that toltrazuril is effective for the treatment of coccidiosis due to E. bovis and E. zuernii in calves and enables the development of a normal or enhanced immune response to infection. The Author(s), 2011 Jonsson Nicholas College of Veterinary, Medical and Life Sciences, University of Glasgow, Bearsden, Scotland, UK aut Piper Emily School of Veterinary Science, University of Queensland, Brisbane, Australia aut Gray Christian School of Veterinary Science, University of Queensland, Brisbane, Australia aut Deniz Abdulkerim Bayer Animal Health, Leverkusen, Germany aut Constantinoiu Constantin School of Veterinary and Biomedical Sciences, James Cook University, Townsville, Australia aut Parasitology Research Springer-Verlag 109(2011-08-01), 113-128 0932-0113 109<113 2011 109 436 https://doi.org/10.1007/s00436-011-2408-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2408-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jonsson Nicholas College of Veterinary, Medical and Life Sciences, University of Glasgow, Bearsden, Scotland, UK aut NATIONALLICENCE 700 1- Piper Emily School of Veterinary Science, University of Queensland, Brisbane, Australia aut NATIONALLICENCE 700 1- Gray Christian School of Veterinary Science, University of Queensland, Brisbane, Australia aut NATIONALLICENCE 700 1- Deniz Abdulkerim Bayer Animal Health, Leverkusen, Germany aut NATIONALLICENCE 700 1- Constantinoiu Constantin School of Veterinary and Biomedical Sciences, James Cook University, Townsville, Australia aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109(2011-08-01), 113-128 0932-0113 109<113 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer