caa a22 4500 445340703 CHVBK 20180317142811.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00436-011-2360-1 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2360-1 A study on the prevalence of a tick-transmitted pathogen, Theileria annulata , and hematological profile of cattle from Southern Punjab (Pakistan) [Elektronische Daten] [S. Shahnawaz, M. Ali, M. Aslam, R. Fatima, Z. Chaudhry, M. Hassan, M. Ali, Furhan Iqbal] The present study was carried out to determine the prevalence of Theileria annulata in large ruminants in Southern Punjab (Pakistan). Blood samples were collected from 144 large ruminants, consisting of 105 cattle and 39 buffaloes, from six districts of Southern Punjab including Multan, Layyah, Muzaffar Garh, Bhakar, Bahawalnagar, and Vehari. Data on the characteristics of the animals and herds were collected through questionnaires. The age of animals (P = 0.02), presence of ticks on animals (P = 0.02), and presence of ticks on dogs associated with herds (P = 0.05) were among the major risk factors involved in the spread of tropical theileriosis in the study area. Two different parasite detection techniques, PCR amplification and screening of Giemsa-stained slides, were compared, and it was found that PCR amplification is a more sensitive tool (19% parasite detection) as compared to smear scanning (3% parasite detection) for the detection of T. annulata. Twenty eight out of 144 animals produced the 721-bp fragment specific for T. annulata from five out of six sampling districts. Different blood (hemoglobin, glucose) and serum (ALT, AST, LDH, cholesterol) parameters of calves and cattle were measured and compared between parasite-positive and parasite-negative samples to assess the effect of T. annulata on the blood and serological profile of infected animals. Springer-Verlag, 2011 Shahnawaz S. Zoology Division, Institute of Pure and Applied Biology, Bahauddin Zakariya University, 60800, Multan, Pakistan aut Ali M. Faculty of Veterinary Sciences, Bahauddin Zakariya University, 60800, Multan, Pakistan aut Aslam M. Institute of Biotechnology, Bahauddin Zakariya University, 60800, Multan, Pakistan aut Fatima R. Zoology Division, Institute of Pure and Applied Biology, Bahauddin Zakariya University, 60800, Multan, Pakistan aut Chaudhry Z. Faculty of Veterinary Sciences, Bahauddin Zakariya University, 60800, Multan, Pakistan aut Hassan M. Department of Statistics, Bahauddin Zakariya University, 60800, Multan, Pakistan aut Ali M. Institute of Biotechnology, Bahauddin Zakariya University, 60800, Multan, Pakistan aut Iqbal Furhan Zoology Division, Institute of Pure and Applied Biology, Bahauddin Zakariya University, 60800, Multan, Pakistan aut Parasitology Research Springer-Verlag 109/4(2011-10-01), 1155-1160 0932-0113 109:4<1155 2011 109 436 https://doi.org/10.1007/s00436-011-2360-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2360-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shahnawaz S. Zoology Division, Institute of Pure and Applied Biology, Bahauddin Zakariya University, 60800, Multan, Pakistan aut NATIONALLICENCE 700 1- Ali M. Faculty of Veterinary Sciences, Bahauddin Zakariya University, 60800, Multan, Pakistan aut NATIONALLICENCE 700 1- Aslam M. Institute of Biotechnology, Bahauddin Zakariya University, 60800, Multan, Pakistan aut NATIONALLICENCE 700 1- Fatima R. Zoology Division, Institute of Pure and Applied Biology, Bahauddin Zakariya University, 60800, Multan, Pakistan aut NATIONALLICENCE 700 1- Chaudhry Z. Faculty of Veterinary Sciences, Bahauddin Zakariya University, 60800, Multan, Pakistan aut NATIONALLICENCE 700 1- Hassan M. Department of Statistics, Bahauddin Zakariya University, 60800, Multan, Pakistan aut NATIONALLICENCE 700 1- Ali M. Institute of Biotechnology, Bahauddin Zakariya University, 60800, Multan, Pakistan aut NATIONALLICENCE 700 1- Iqbal Furhan Zoology Division, Institute of Pure and Applied Biology, Bahauddin Zakariya University, 60800, Multan, Pakistan aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/4(2011-10-01), 1155-1160 0932-0113 109:4<1155 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer