caa a22 4500 44534072X CHVBK 20180317142811.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00436-011-2341-4 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2341-4 Fasciola hepatica [Elektronische Daten] time-dependent disruption of spermatogenesis following in vivo treatment with triclabendazole [Emma Toner, Gerard Brennan, Robert Hanna, Hillary Edgar, Ian Fairweather] Sheep infected with the Cullompton isolate of Fasciola hepatica were treated with triclabendazole at a concentration of 10mg/kg at 12weeks post-infection. Adult flukes were recovered from the liver and, where present, from the gall bladder at 48, 72 and 96h post-treatment (pt). Gross changes to the spermatogenic cells of the testis were examined by histology and ultrastructural alterations were visualised via transmission electron microscopy. Disruption was progressive in nature, with the testis tubules becoming shrunken, vacuolated and gradually more denuded of cellular content over the 96-h time period. From 48hpt, the number of primary and secondary spermatogonia decreased and multinucleate spermatogonial cells were frequent. Later, developmental stages were uncommon, giving rise to much empty space within the tubules. By 72hpt, the tubules contained many apoptotic and degraded cells and had an extremely disorganised appearance. At 96hpt, the tubules were almost completely empty, with the exception of the remains of degraded spermatogenic cells. These results indicate that triclabendazole severely disrupts spermatogenesis in the liver fluke from 48hpt in vivo. Springer-Verlag, 2011 Toner Emma Parasite Therapeutics Research Group, School of Biological Sciences, Medical Biology Centre, The Queen's University of Belfast, 97 Lisburn Road, BT9 7BL, Belfast, UK aut Brennan Gerard Parasite Therapeutics Research Group, School of Biological Sciences, Medical Biology Centre, The Queen's University of Belfast, 97 Lisburn Road, BT9 7BL, Belfast, UK aut Hanna Robert Veterinary Sciences Division, Agri-Food and Biosciences Institute, Stormont, BT4 3SD, Belfast, UK aut Edgar Hillary Veterinary Sciences Division, Agri-Food and Biosciences Institute, Stormont, BT4 3SD, Belfast, UK aut Fairweather Ian Parasite Therapeutics Research Group, School of Biological Sciences, Medical Biology Centre, The Queen's University of Belfast, 97 Lisburn Road, BT9 7BL, Belfast, UK aut Parasitology Research Springer-Verlag 109/4(2011-10-01), 1035-1043 0932-0113 109:4<1035 2011 109 436 https://doi.org/10.1007/s00436-011-2341-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2341-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Toner Emma Parasite Therapeutics Research Group, School of Biological Sciences, Medical Biology Centre, The Queen's University of Belfast, 97 Lisburn Road, BT9 7BL, Belfast, UK aut NATIONALLICENCE 700 1- Brennan Gerard Parasite Therapeutics Research Group, School of Biological Sciences, Medical Biology Centre, The Queen's University of Belfast, 97 Lisburn Road, BT9 7BL, Belfast, UK aut NATIONALLICENCE 700 1- Hanna Robert Veterinary Sciences Division, Agri-Food and Biosciences Institute, Stormont, BT4 3SD, Belfast, UK aut NATIONALLICENCE 700 1- Edgar Hillary Veterinary Sciences Division, Agri-Food and Biosciences Institute, Stormont, BT4 3SD, Belfast, UK aut NATIONALLICENCE 700 1- Fairweather Ian Parasite Therapeutics Research Group, School of Biological Sciences, Medical Biology Centre, The Queen's University of Belfast, 97 Lisburn Road, BT9 7BL, Belfast, UK aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/4(2011-10-01), 1035-1043 0932-0113 109:4<1035 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer