caa a22 4500 445340762 CHVBK 20180317142811.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00436-011-2350-3 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2350-3 Kinetics of capture and infection of infective larvae of trichostrongylides and free-living nematodes Panagrellus sp. by Duddingtonia flagrans [Elektronische Daten] [Daniela da Cruz, Flávia Araújo, Marcelo Molento, Renato DaMatta, Clóvis de Paula Santos] Duddingtonia flagrans, a nematode-trapping fungus, has been investigated as an agent for biological control against infective larvae of gastrointestinal nematode parasites of production animals. The initial process of nematode-trapping fungi infection is based on an interaction between the trap structure of the fungus and the surface of the nematode cuticle. This report investigates by light and scanning electron microscopy the kinetics of capture and infection during the interaction of D. flagrans with the infective larvae (L3) of trichostrongylides and the free-living nematode Panagrellus sp. D. flagrans was cultivated for 7days in a Petri dish containing agar-water. L3 and Panagrellus sp. were inoculated in the Petri dishes and the samples consisting of agar-L3-fungi and agar-Panagrellus sp.-fungi were collected after 10, 20, 30, 40, 50, 60, and 70min and 3, 4, 5, 10, 15, 20, and 25h of interaction. All samples were observed by light microscopy. The samples with 1, 5, 15, and 25h of interaction were also analyzed by scanning electron microscopy. The interaction was monitored up to 25h. An initial differentiation of predation structures was observed after 30min of interaction. The presence of traps and of captured L3 or Panagrellus sp. occurred after 70min. The live captured nematodes were observed up to 3h of interaction. However, after 4h, all Panagrellus sp. were dead. It took 15h of interaction for the fungus to invade the L3, and the presence of hyphae inside the nematode near the region of penetration was evident. At this time, the hyphae had filled the whole body of Panagrellus sp. The complete occupation of the body of L3 occurred at 20h of interaction and with 25h the nematode was completely damaged except for the cuticle. Although the double cuticle of L3 slows the penetration of D. flagrans, it was possible to verify that the process of trap formation and capture occurs quickly when both nematodes were tested, suggesting that the organisms would eventually be killed once in contact with the fungi encouraging the use of the fungus as a biological control agent. Springer-Verlag, 2011 da Cruz Daniela Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Avenida Alberto Lamego, 2000, Parque Califórnia, Campos dos Goytacazes, CEP 28013-602, Rio de Janeiro, Brazil aut Araújo Flávia Laboratório de Doenças Parasitárias, Departamento de Medicina Veterinária, Universidade Federal do Paraná, Rua dos Funcionários, 1540, Cabral, CEP: 80035-050, Curitiba, PR, Brazil aut Molento Marcelo Laboratório de Doenças Parasitárias, Departamento de Medicina Veterinária, Universidade Federal do Paraná, Rua dos Funcionários, 1540, Cabral, CEP: 80035-050, Curitiba, PR, Brazil aut DaMatta Renato Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Avenida Alberto Lamego, 2000, Parque Califórnia, Campos dos Goytacazes, CEP 28013-602, Rio de Janeiro, Brazil aut de Paula Santos Clóvis Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Avenida Alberto Lamego, 2000, Parque Califórnia, Campos dos Goytacazes, CEP 28013-602, Rio de Janeiro, Brazil aut Parasitology Research Springer-Verlag 109/4(2011-10-01), 1085-1091 0932-0113 109:4<1085 2011 109 436 https://doi.org/10.1007/s00436-011-2350-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2350-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- da Cruz Daniela Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Avenida Alberto Lamego, 2000, Parque Califórnia, Campos dos Goytacazes, CEP 28013-602, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Araújo Flávia Laboratório de Doenças Parasitárias, Departamento de Medicina Veterinária, Universidade Federal do Paraná, Rua dos Funcionários, 1540, Cabral, CEP: 80035-050, Curitiba, PR, Brazil aut NATIONALLICENCE 700 1- Molento Marcelo Laboratório de Doenças Parasitárias, Departamento de Medicina Veterinária, Universidade Federal do Paraná, Rua dos Funcionários, 1540, Cabral, CEP: 80035-050, Curitiba, PR, Brazil aut NATIONALLICENCE 700 1- DaMatta Renato Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Avenida Alberto Lamego, 2000, Parque Califórnia, Campos dos Goytacazes, CEP 28013-602, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- de Paula Santos Clóvis Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Avenida Alberto Lamego, 2000, Parque Califórnia, Campos dos Goytacazes, CEP 28013-602, Rio de Janeiro, Brazil aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/4(2011-10-01), 1085-1091 0932-0113 109:4<1085 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer