caa a22 4500 445340797 CHVBK 20180317142812.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00436-011-2364-x doi (NATIONALLICENCE)springer-10.1007/s00436-011-2364-x In silico characterization and comparative genomic analysis of the Culex quinquefasciatus glutathione S -transferase (GST) supergene family [Elektronische Daten] [B. Niranjan Reddy, G. Prasad, K. Raghavendra] The glutathione S-transferases (GSTs) are phase II class of detoxification enzymes that are involved both directly and indirectly in insecticide resistance mechanisms. The Culex quinquefasciatus GST superfamily was analyzed by utilizing the public domain Culex genome sequence. In total, 35 cytosolic (seven classes) and 5 microsomal putatively active GSTs were retrieved, classified, and annotated. The study revealed the presence of three unclassified GSTs. Of 35 cytosolic GSTs, 65% contributed by insect specific Delta-Epsilon classes. Gene cluster analysis revealed that most of the genes of Delta, Epsilon, and Theta classes were organized into gene clusters. The gene organization analysis revealed the dominance of phase "0” introns in the Culex GST family. The studies on intron loss and gain events revealed that the Delta GSTs have experienced a higher number of loss and gains during their evolution. A positive correlation was observed between the phylogenetic relationship of members of the GST superfamily and their corresponding exon-intron organization. In addition, the genes within the gene clusters revealed the monophyletic phylogenetic relationship implying the importance of gene duplication events in the gene families' evolution. Finally, the comparative genomic analysis has shown a complex evolutionary scenario associated with the GST supergene family evolution in insects. Springer-Verlag, 2011 Niranjan Reddy B. Vector Control Division, National Institute of Malaria Research (ICMR), Sector 8, Dwarka, 110077, New Delhi, India aut Prasad G. School Of Studies in Biotechnology, Jiwaji University, Gwalior, MP, India aut Raghavendra K. Vector Control Division, National Institute of Malaria Research (ICMR), Sector 8, Dwarka, 110077, New Delhi, India aut Parasitology Research Springer-Verlag 109/4(2011-10-01), 1165-1177 0932-0113 109:4<1165 2011 109 436 https://doi.org/10.1007/s00436-011-2364-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2364-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Niranjan Reddy B. Vector Control Division, National Institute of Malaria Research (ICMR), Sector 8, Dwarka, 110077, New Delhi, India aut NATIONALLICENCE 700 1- Prasad G. School Of Studies in Biotechnology, Jiwaji University, Gwalior, MP, India aut NATIONALLICENCE 700 1- Raghavendra K. Vector Control Division, National Institute of Malaria Research (ICMR), Sector 8, Dwarka, 110077, New Delhi, India aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/4(2011-10-01), 1165-1177 0932-0113 109:4<1165 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer