caa a22 4500 445340819 CHVBK 20180317142812.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00436-011-2342-3 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2342-3 Evaluation of DNA extraction kits for molecular diagnosis of human Blastocystis subtypes from fecal samples [Elektronische Daten] [Hisao Yoshikawa, Funda Dogruman-AI, Songul Turk, Semra Kustimur, Neriman Balaban, Nedim Sultan] Blastocystis sp. is now recognized as one of the most common intestinal parasite in human fecal examinations. Recently, PCR-based diagnostic methods of Blastocystis infection using direct DNA extraction from fresh fecal samples with commercially available kits are reported. Several kits have been developed, but little has been done in comparing the detective sensitivity between PCR methods using the commercial kits. In this study, we compared the detective sensitivity among five commercially available kits (MagNA Pure LC DNA Isolation Kit I, Roche; QuickGene SP Kit DNA, FujiFilm; NucleoSpin Plant II, Macherey-Nagel; QIAamp DNA Stool Mini Kit, Qiagen; ZR Fecal DNA Kit, Zymo Research) and fecal culture method. In a preliminary test, the DNA isolated with two kits (FujiFilm and Macherey-Nagel) showed negative PCR, while the other three kits showed positive PCR. Then, DNA from 50 clinical samples that was Blastocystis-positive in the examination of fecal culture method were isolated with the three kits and 1.1kbp SSU rRNA gene was detected with PCR. The positive rates of the three kits (Roche, Qiagen, and Zymo Research) were 10, 48 and 94%, respectively. The present study indicated that there is different detective sensitivity among the commercial kits, and fecal culture method is superior in detection rate and cost performance than DNA-elution kits for diagnosis of Blastocystis sp. subtypes. Springer-Verlag, 2011 Yoshikawa Hisao Department of Biological Sciences, Faculty of Science, Nara Women's University, 630-8605, Nara, Japan aut Dogruman-AI Funda Department of Medical Microbiology, Gazi University Faculty of Medicine, Ankara, Turkey aut Turk Songul Department of Medical Microbiology, Gazi University Faculty of Medicine, Ankara, Turkey aut Kustimur Semra Department of Medical Microbiology, Gazi University Faculty of Medicine, Ankara, Turkey aut Balaban Neriman Second Microbiology Laboratory, Ankara Numune Education and Research Hospital, Ankara, Turkey aut Sultan Nedim Department of Medical Microbiology, Gazi University Faculty of Medicine, Ankara, Turkey aut Parasitology Research Springer-Verlag 109/4(2011-10-01), 1045-1050 0932-0113 109:4<1045 2011 109 436 https://doi.org/10.1007/s00436-011-2342-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2342-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yoshikawa Hisao Department of Biological Sciences, Faculty of Science, Nara Women's University, 630-8605, Nara, Japan aut NATIONALLICENCE 700 1- Dogruman-AI Funda Department of Medical Microbiology, Gazi University Faculty of Medicine, Ankara, Turkey aut NATIONALLICENCE 700 1- Turk Songul Department of Medical Microbiology, Gazi University Faculty of Medicine, Ankara, Turkey aut NATIONALLICENCE 700 1- Kustimur Semra Department of Medical Microbiology, Gazi University Faculty of Medicine, Ankara, Turkey aut NATIONALLICENCE 700 1- Balaban Neriman Second Microbiology Laboratory, Ankara Numune Education and Research Hospital, Ankara, Turkey aut NATIONALLICENCE 700 1- Sultan Nedim Department of Medical Microbiology, Gazi University Faculty of Medicine, Ankara, Turkey aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/4(2011-10-01), 1045-1050 0932-0113 109:4<1045 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer