caa a22 4500 445340908 CHVBK 20180317142812.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00436-011-2345-0 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2345-0 An immunohistochemical study in cases with usual and unusual clinicopathological findings of canine visceral leishmaniosis [Elektronische Daten] [Nihat Toplu, Ahmet Aydogan] The present study describes pathologic findings and immunohistochemical diagnosis of canine visceral leishmaniasis (CVL) in 22 dogs who died naturally in the Aegean region of Turkey. At necropsy, lymphadenomegaly, hepatosplenomegaly, hepatic, and nephrosclerotic lesions were conspicuous. Histopathologically, chronic inflammatory reactions of the spleen, lymph nodes, bone marrow, liver, and skin were marked findings. Cytological and histological examinations showed macrophages loaded with Leishmania amastigotes in these organs. Immunohistochemistry revealed that immunolabeling of amastigotes and/or parasite antigen, especially in the lymph nodes, spleen, bone marrow, liver, and skin, and occasionally, in the kidneys, intestines and lungs. Our laboratory results showed that immunohistochemistry should be included, along with cytological and histological examinations, in the diagnosis of CVL. Springer-Verlag, 2011 Toplu Nihat Department of Pathology, Faculty of Veterinary Medicine, Adnan Menderes University, 09016-Isikli, Aydin, Turkey aut Aydogan Ahmet Department of Pathology, Faculty of Veterinary Medicine, Adnan Menderes University, 09016-Isikli, Aydin, Turkey aut Parasitology Research Springer-Verlag 109/4(2011-10-01), 1051-1057 0932-0113 109:4<1051 2011 109 436 https://doi.org/10.1007/s00436-011-2345-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2345-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Toplu Nihat Department of Pathology, Faculty of Veterinary Medicine, Adnan Menderes University, 09016-Isikli, Aydin, Turkey aut NATIONALLICENCE 700 1- Aydogan Ahmet Department of Pathology, Faculty of Veterinary Medicine, Adnan Menderes University, 09016-Isikli, Aydin, Turkey aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/4(2011-10-01), 1051-1057 0932-0113 109:4<1051 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer