caa a22 4500 445340967 CHVBK 20180317142812.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00436-011-2365-9 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2365-9 Prodigiosin produced by Serratia marcescens NMCC46 as a mosquito larvicidal agent against Aedes aegypti and Anopheles stephensi [Elektronische Daten] [Chandrashekhar Patil, Satish Patil, Bipinchandra Salunke, Rahul Salunkhe] Microbial control agents offer alternatives to chemical pest control as they can be more selective than chemical insecticides. The present study evaluates the mosquito larvicidal potential of microbial pigment prodigiosin produced by Serratia marcescens NMCC46 against Aedes aegypti and Anopheles stephensi. The pigment of S. marcescens NMCC46 was extracted after 24h from mannitol containing nutrient broth media. The effects of crude extracted pigment on the growth, survival, development, and other life cycle aspects were studied. The LC50 and LC90 values of second, third, and fourth instars of A. aegypti (LC50 = 41.65, 139.51, 103.95; LC90 = 117.81, 213.68, 367.82) and A. stephensi (LC50 = 51.12, 105.52, 133.07; LC90 = 134.81, 204.45, 285.35) were determined. At higher concentration (500ppm), mortality starts within first 6h of exposure. More than 50% mortality occurs within the first 24h. The overall observed effects against A. aegypti and A. stephensi larvae after 48h were increasing percent survival larvae, survival pupation, adult emergence with decreasing crude pigment extract concentration. These ensure that the resultant mosquito population reduction is substantial even where the larvicidal potential is minimal. The UV (λ max = 536nm), TLC (Rf = 0.9), HPLC, and FTIR analysis of crude pigment shows the presence of prodigiosin as active compound. Thus, the active compound produced by this species would be more useful against vectors responsible for diseases of public health importance. This is the first report on mosquito larvicidal activity of prodigiosin produced by Serratia species. Springer-Verlag, 2011 Patil Chandrashekhar School of Life Sciences, North Maharashtra University, Post Box - 80, 425001, Jalgaon, Maharashtra, India aut Patil Satish School of Life Sciences, North Maharashtra University, Post Box - 80, 425001, Jalgaon, Maharashtra, India aut Salunke Bipinchandra School of Life Sciences, North Maharashtra University, Post Box - 80, 425001, Jalgaon, Maharashtra, India aut Salunkhe Rahul School of Life Sciences, North Maharashtra University, Post Box - 80, 425001, Jalgaon, Maharashtra, India aut Parasitology Research Springer-Verlag 109/4(2011-10-01), 1179-1187 0932-0113 109:4<1179 2011 109 436 https://doi.org/10.1007/s00436-011-2365-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2365-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Patil Chandrashekhar School of Life Sciences, North Maharashtra University, Post Box - 80, 425001, Jalgaon, Maharashtra, India aut NATIONALLICENCE 700 1- Patil Satish School of Life Sciences, North Maharashtra University, Post Box - 80, 425001, Jalgaon, Maharashtra, India aut NATIONALLICENCE 700 1- Salunke Bipinchandra School of Life Sciences, North Maharashtra University, Post Box - 80, 425001, Jalgaon, Maharashtra, India aut NATIONALLICENCE 700 1- Salunkhe Rahul School of Life Sciences, North Maharashtra University, Post Box - 80, 425001, Jalgaon, Maharashtra, India aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/4(2011-10-01), 1179-1187 0932-0113 109:4<1179 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer