caa a22 4500 445341009 CHVBK 20180317142812.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00436-011-2376-6 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2376-6 Molecular characterization of Hepatozoon sp. in cats from São Luís Island, Maranhão, Northeastern Brazil [Elektronische Daten] [Caroline de Bortoli, Marcos André, Maria do Braga, Rosangela Machado] Few molecular studies have been done concerning the molecular characterization of Hepatozoon species among domestic and wild felids. The present work aimed to characterize molecularly the presence of Hepatozoon sp. DNA in cat blood samples from São Luís Island, Maranhão state, Northeastern Brazil. EDTA-whole blood samples were collected from 200 domestic cats with outdoor and wood areas access from São Luís, Maranhão, Brazil. Each sample of extracted DNA was used as a template in PCR reactions aiming to amplify a partial sequence of 18S rRNA of Hepatozoon spp. We also performed sequence alignment to establish the identity of the parasite species infecting these animals using DNA sequences based on 18S rRNA. From 200 sampled cats, Hepatozoon DNA was only found in one animal (0.5%). The found Hepatozoon DNA showed 97% of identity with Hemobartonella felis isolates 1 and 2 from Spain. When analyzing the phylogenetic tree, the found Hepatozoon DNA was in the same clade than H. felis isolates. Our findings suggest that more than one species of Hepatozoon could infect felids in Brazil. Springer-Verlag, 2011 de Bortoli Caroline Universidade Estadual Paulista, UNESP, Jaboticabal, SP, Brazil aut André Marcos Universidade Estadual Paulista, UNESP, Jaboticabal, SP, Brazil aut Braga Maria do Universidade Estadual Paulista, UNESP, Jaboticabal, SP, Brazil aut Machado Rosangela Universidade Estadual Paulista, UNESP, Jaboticabal, SP, Brazil aut Parasitology Research Springer-Verlag 109/4(2011-10-01), 1189-1192 0932-0113 109:4<1189 2011 109 436 https://doi.org/10.1007/s00436-011-2376-6 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2376-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de Bortoli Caroline Universidade Estadual Paulista, UNESP, Jaboticabal, SP, Brazil aut NATIONALLICENCE 700 1- André Marcos Universidade Estadual Paulista, UNESP, Jaboticabal, SP, Brazil aut NATIONALLICENCE 700 1- Braga Maria do Universidade Estadual Paulista, UNESP, Jaboticabal, SP, Brazil aut NATIONALLICENCE 700 1- Machado Rosangela Universidade Estadual Paulista, UNESP, Jaboticabal, SP, Brazil aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/4(2011-10-01), 1189-1192 0932-0113 109:4<1189 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer