caa a22 4500 445341025 CHVBK 20180317142812.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00436-011-2385-5 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2385-5 Which McMaster egg counting technique is the most reliable? [Elektronische Daten] [Jaroslav Vadlejch, Miloslav Petrtýl, Igor Zaichenko, Zuzana Čadková, Ivana Jankovská, Iva Langrová, Milan Moravec] This study focuses on the comparison of three selected modifications of the McMaster counting technique, namely the McMaster method modified by Wetzel (W) and Zajíček (Z), as well as the concentration McMaster technique according to Roepstorff and Nansen (R&N). These modifications differ in the weights of faeces examined (W, 2g/Z, 1g/R&N, 4g), flotation solutions (W, NaCl/Z, MgSO4 + Na2S2O3/R&N, NaCl + glucose), centrifugation (W, none/Z, 2,000RPM for 2min and 2,000RPM for 1min/R&N, 1,200RPM for 5min), number of McMaster chambers investigated (W, 3/Z, 2/R&N, 2), and multiplication factors used (W, 67/Z, 33/R&N, 20). To investigate the sensitivity and reliability of these methods, nematode eggs (Teladorsagia circumcincta) were used. Parasite elements are distributed through negative binomial distribution in naturally infected host faeces, and the number of parasite elements in a given amount of faeces sample is unknown to man. Therefore, we decided to prepare the exact number of eggs which were added to the parasite negative faeces; the faecal sample was then investigated. From this perspective, this is the first time a comparison of the McMaster methods has been so accurately investigated. This approach allows us to evaluate the real sensitivity and reliability of the tested method. As the findings of this study indicate, the highest sensitivity and reliability were obtained using the Roepstorff and Nansen modification. This McMaster modification is able to detect 20 eggs per sample (in 70% of samples). Concentrations of 200 and 500 eggs can be found in almost 100% of samples. Moreover, this method is simple, cheap and fast. For these reasons, we can recommend this method for routine veterinary practice. Springer-Verlag, 2011 Vadlejch Jaroslav Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 957, 165 21, Prague 6, Suchdol, Czech Republic aut Petrtýl Miloslav Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 957, 165 21, Prague 6, Suchdol, Czech Republic aut Zaichenko Igor Department of Therapy and Pharmacology, Veterinary Medicine Faculty, Stavropol State Agrarian University, Zootechnichesky 12, 355 917, Stavropol, Russia aut Čadková Zuzana Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 957, 165 21, Prague 6, Suchdol, Czech Republic aut Jankovská Ivana Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 957, 165 21, Prague 6, Suchdol, Czech Republic aut Langrová Iva Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 957, 165 21, Prague 6, Suchdol, Czech Republic aut Moravec Milan Department of Environment, Hydroprojekt CZ, a. s., Táborská 31, 140 16, Prague 4, Nusle, Czech Republic aut Parasitology Research Springer-Verlag 109/5(2011-11-01), 1387-1394 0932-0113 109:5<1387 2011 109 436 https://doi.org/10.1007/s00436-011-2385-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2385-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vadlejch Jaroslav Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 957, 165 21, Prague 6, Suchdol, Czech Republic aut NATIONALLICENCE 700 1- Petrtýl Miloslav Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 957, 165 21, Prague 6, Suchdol, Czech Republic aut NATIONALLICENCE 700 1- Zaichenko Igor Department of Therapy and Pharmacology, Veterinary Medicine Faculty, Stavropol State Agrarian University, Zootechnichesky 12, 355 917, Stavropol, Russia aut NATIONALLICENCE 700 1- Čadková Zuzana Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 957, 165 21, Prague 6, Suchdol, Czech Republic aut NATIONALLICENCE 700 1- Jankovská Ivana Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 957, 165 21, Prague 6, Suchdol, Czech Republic aut NATIONALLICENCE 700 1- Langrová Iva Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 957, 165 21, Prague 6, Suchdol, Czech Republic aut NATIONALLICENCE 700 1- Moravec Milan Department of Environment, Hydroprojekt CZ, a. s., Táborská 31, 140 16, Prague 4, Nusle, Czech Republic aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/5(2011-11-01), 1387-1394 0932-0113 109:5<1387 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer