caa a22 4500 445341084 CHVBK 20180317142813.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00436-011-2381-9 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2381-9 Prokaryotic expression and identification of 3-1E gene of merozoite surface antigen of Eimeria acervulina [Elektronische Daten] [Yuelan Zhao, Chengmin Wang, Yanmin Lu, Said Amer, Ping Xu, Jianyong Wang, Junxia Lu, Yongzhan Bao, Bolin Deng, Hongxuan He, Jianhua Qin] Eimeria acervulina was isolated from chicken at Hebei province, China. The gene of merozoite surface antigen 3-1E was amplified and cloned into pET28a(+) vector and then transformed into Escherichia coli BL21 strain. Results showed that 3-1E fusion protein band of about 22kDa was identified by SDS-PAGE. Western blot analysis indicated that the recombinant protein specifically reacted with E. acervulina polyclonal antibody. Springer-Verlag, 2011 Zhao Yuelan College of Chinese Traditional Veterinary Science, Hebei Agriculture University, Dingzhou, China aut Wang Chengmin National Research Center for Wildlife Borne Diseases, Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China aut Lu Yanmin National Research Center for Wildlife Borne Diseases, Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China aut Amer Said Department of Zoology, Faculty of Science, Kafr El-Sheikh University, 33516, Kafr El-Sheikh, Egypt aut Xu Ping National Research Center for Wildlife Borne Diseases, Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China aut Wang Jianyong College of Chinese Traditional Veterinary Science, Hebei Agriculture University, Dingzhou, China aut Lu Junxia College of Animal Science and Technology, Hebei Agriculture University, Baoding, China aut Bao Yongzhan College of Animal Science and Technology, Hebei Agriculture University, Baoding, China aut Deng Bolin Animal Husbandry and Veterinary Station of Beijing, 100101, Beijing, China aut He Hongxuan National Research Center for Wildlife Borne Diseases, Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China aut Qin Jianhua College of Animal Science and Technology, Hebei Agriculture University, Baoding, China aut Parasitology Research Springer-Verlag 109/5(2011-11-01), 1361-1365 0932-0113 109:5<1361 2011 109 436 https://doi.org/10.1007/s00436-011-2381-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2381-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Yuelan College of Chinese Traditional Veterinary Science, Hebei Agriculture University, Dingzhou, China aut NATIONALLICENCE 700 1- Wang Chengmin National Research Center for Wildlife Borne Diseases, Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Lu Yanmin National Research Center for Wildlife Borne Diseases, Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Amer Said Department of Zoology, Faculty of Science, Kafr El-Sheikh University, 33516, Kafr El-Sheikh, Egypt aut NATIONALLICENCE 700 1- Xu Ping National Research Center for Wildlife Borne Diseases, Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Wang Jianyong College of Chinese Traditional Veterinary Science, Hebei Agriculture University, Dingzhou, China aut NATIONALLICENCE 700 1- Lu Junxia College of Animal Science and Technology, Hebei Agriculture University, Baoding, China aut NATIONALLICENCE 700 1- Bao Yongzhan College of Animal Science and Technology, Hebei Agriculture University, Baoding, China aut NATIONALLICENCE 700 1- Deng Bolin Animal Husbandry and Veterinary Station of Beijing, 100101, Beijing, China aut NATIONALLICENCE 700 1- He Hongxuan National Research Center for Wildlife Borne Diseases, Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Qin Jianhua College of Animal Science and Technology, Hebei Agriculture University, Baoding, China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/5(2011-11-01), 1361-1365 0932-0113 109:5<1361 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer