caa a22 4500 445341092 CHVBK 20180317142813.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00436-011-2370-z doi (NATIONALLICENCE)springer-10.1007/s00436-011-2370-z Measurement of naturally acquired humoral immune responses against the C-terminal region of the Plasmodium vivax MSP1 protein using protein arrays [Elektronische Daten] [Jun-Hu Chen, Yue Wang, Kwon-Soo Ha, Feng Lu, In-Bum Suh, Chae Lim, Jeong Park, Satoru Takeo, Takafumi Tsuboi, Eun-Taek Han] Protein arrays are powerful tools for antibody profiling and vaccine development against infectious agents. In the previous report, we successfully applied an antibody-based protein array for immunoprofiling of Plasmodium vivax infection. Herein, we developed a Ni-NTA surface based protein array to detect immune responses against the recombinant C-terminal region (19 and 42kDa) of the P. vivax merozoite surface protein 1 (PvMSP1-19 and -42) from sera of vivax malaria patients. The PvMSP1-19 arrays detected P. vivax in 112 of 130 (86.2%; 95% CI, 83.2-89.2%) microscopically positive samples and 2 false positives were obtained among 100 sera samples from healthy subjects (2.0%; 95% CI, 0.6-3.4%). These results were in concordance with results of enzyme-linked immunosorbent assays (ELISA). Kappa values represented excellent agreement for the recombinant PvMSP1-19 protein against sera samples as measured by protein arrays and ELISA (Kappa = 0.904, 95% CI: 0.849-0.960). The PvMSP1-42 protein arrays detected antibody response in 100 of 130 microscopically positive samples (76.9%; 95% CI, 72.4-86.8%) and 8 false positives were obtained in 100 healthy subjects (8.0%; 95% CI, 2.7-13.3%). There is no significant difference between the fluorescent intensity of antibody response to PvMSP1-19 and PvMSP1-42 in the positive sera samples (P > 0.05). The novel protein array platform may be used for profiling naturally acquired humoral immune responses to P. vivax infection. Springer-Verlag, 2011 Chen Jun-Hu Department of Parasitology and Institute of Medical Science, Kangwon National University School of Medicine, Hyoja2-dong, 200-701, Chunchon, Kangwon-do, Republic of Korea aut Wang Yue Department of Parasitology and Institute of Medical Science, Kangwon National University School of Medicine, Hyoja2-dong, 200-701, Chunchon, Kangwon-do, Republic of Korea aut Ha Kwon-Soo Department of Molecular and Cellular Biochemistry and Institute of Medical Science, Kangwon National University School of Medicine, 200-701, Chunchon, Republic of Korea aut Lu Feng Department of Parasitology and Institute of Medical Science, Kangwon National University School of Medicine, Hyoja2-dong, 200-701, Chunchon, Kangwon-do, Republic of Korea aut Suh In-Bum Department of Laboratory Medicine and Institute of Medical Science, Kangwon National University School of Medicine, 200-701, Chunchon, Republic of Korea aut Lim Chae Department of Laboratory Medicine, College of Medicine, Korea University, Seoul, Republic of Korea aut Park Jeong Department of Anatomy and Cell Biology, Kangwon National University School of Medicine, 200-701, Chunchon, Republic of Korea aut Takeo Satoru Cell-Free Science and Technology Research Center, Ehime University, 790-8577, Matsuyama, Ehime, Japan aut Tsuboi Takafumi Cell-Free Science and Technology Research Center, Ehime University, 790-8577, Matsuyama, Ehime, Japan aut Han Eun-Taek Department of Parasitology and Institute of Medical Science, Kangwon National University School of Medicine, Hyoja2-dong, 200-701, Chunchon, Kangwon-do, Republic of Korea aut Parasitology Research Springer-Verlag 109/5(2011-11-01), 1259-1266 0932-0113 109:5<1259 2011 109 436 https://doi.org/10.1007/s00436-011-2370-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2370-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Jun-Hu Department of Parasitology and Institute of Medical Science, Kangwon National University School of Medicine, Hyoja2-dong, 200-701, Chunchon, Kangwon-do, Republic of Korea aut NATIONALLICENCE 700 1- Wang Yue Department of Parasitology and Institute of Medical Science, Kangwon National University School of Medicine, Hyoja2-dong, 200-701, Chunchon, Kangwon-do, Republic of Korea aut NATIONALLICENCE 700 1- Ha Kwon-Soo Department of Molecular and Cellular Biochemistry and Institute of Medical Science, Kangwon National University School of Medicine, 200-701, Chunchon, Republic of Korea aut NATIONALLICENCE 700 1- Lu Feng Department of Parasitology and Institute of Medical Science, Kangwon National University School of Medicine, Hyoja2-dong, 200-701, Chunchon, Kangwon-do, Republic of Korea aut NATIONALLICENCE 700 1- Suh In-Bum Department of Laboratory Medicine and Institute of Medical Science, Kangwon National University School of Medicine, 200-701, Chunchon, Republic of Korea aut NATIONALLICENCE 700 1- Lim Chae Department of Laboratory Medicine, College of Medicine, Korea University, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Park Jeong Department of Anatomy and Cell Biology, Kangwon National University School of Medicine, 200-701, Chunchon, Republic of Korea aut NATIONALLICENCE 700 1- Takeo Satoru Cell-Free Science and Technology Research Center, Ehime University, 790-8577, Matsuyama, Ehime, Japan aut NATIONALLICENCE 700 1- Tsuboi Takafumi Cell-Free Science and Technology Research Center, Ehime University, 790-8577, Matsuyama, Ehime, Japan aut NATIONALLICENCE 700 1- Han Eun-Taek Department of Parasitology and Institute of Medical Science, Kangwon National University School of Medicine, Hyoja2-dong, 200-701, Chunchon, Kangwon-do, Republic of Korea aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/5(2011-11-01), 1259-1266 0932-0113 109:5<1259 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer