caa a22 4500 445341106 CHVBK 20180317142813.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00436-011-2391-7 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2391-7 Molecular characterization of Hysterothylacium aduncum (Nematoda: Raphidascaridae) from different fish caught off the Tunisian coast based on nuclear ribosomal DNA sequences [Elektronische Daten] [Nabil Amor, Sarra Farjallah, Paolo Merella, Khaled Said, Badreddine Ben Slimane] Larval forms of the genus Hysterothylacium have been previously reported in teleost fish from the North African coasts of central Mediterranean Sea by morphological analysis. In the present study, samples identified morphologically as Hysterothylacium aduncum (n = 62), from Merluccius merluccius, Trachurus mediterraneus and Pagellus erythrinus from different geographical locations of the Tunisian coasts, were genetically characterised by sequences of the first (ITS-1), the 5.8S and second (ITS-2) Internal Transcribed Spacers (ITS) of nuclear ribosomal DNA (rDNA). Comparison of the sequences obtained with those available in public gene databases confirmed that all the samples from the Tunisian coasts belong to a single species, namely H. aduncum. All specimens from the Tunisian coasts showed one indel in position 787 in ITS-2 sequences not reported by any of the previously published sequences from the Atlantic and Pacific Oceans, Adriatic Sea (Mediterranean Sea) and the East Greenland Sea, suggesting the existence of a population-specific pattern exhibiting a low differentiation of this parasite in this area. This is the first molecular characterization of H. aduncum from the Tunisian coasts using ITS rDNA sequences which allows the definition of genetic markers for their unequivocal identification, and provides further biological data on these nematodes in marine fish off the Tunisian coasts, improving the picture of the occurrence of these taxa in the North African coasts of central Mediterranean Sea. Springer-Verlag, 2011 Amor Nabil Unité de Recherche: Génétique, Biodiversité et Valorisation des Bioressources UR/09-30, Institut Supérieur de Biotechnologie de Monastir, Monastir, Tunisia aut Farjallah Sarra Unité de Recherche: Génétique, Biodiversité et Valorisation des Bioressources UR/09-30, Institut Supérieur de Biotechnologie de Monastir, Monastir, Tunisia aut Merella Paolo Sezione di Parassitologia e Malattie Parassitarie Dipartimento di Biologia Animale, Università degli Studi di Sassari, Sardaigne, Italy aut Said Khaled Unité de Recherche: Génétique, Biodiversité et Valorisation des Bioressources UR/09-30, Institut Supérieur de Biotechnologie de Monastir, Monastir, Tunisia aut Ben Slimane Badreddine Institut Supérieur des Sciences et Technologies de l'Environnement de Borj Essedria, Borj Essedria, Tunisia aut Parasitology Research Springer-Verlag 109/5(2011-11-01), 1429-1437 0932-0113 109:5<1429 2011 109 436 https://doi.org/10.1007/s00436-011-2391-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2391-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Amor Nabil Unité de Recherche: Génétique, Biodiversité et Valorisation des Bioressources UR/09-30, Institut Supérieur de Biotechnologie de Monastir, Monastir, Tunisia aut NATIONALLICENCE 700 1- Farjallah Sarra Unité de Recherche: Génétique, Biodiversité et Valorisation des Bioressources UR/09-30, Institut Supérieur de Biotechnologie de Monastir, Monastir, Tunisia aut NATIONALLICENCE 700 1- Merella Paolo Sezione di Parassitologia e Malattie Parassitarie Dipartimento di Biologia Animale, Università degli Studi di Sassari, Sardaigne, Italy aut NATIONALLICENCE 700 1- Said Khaled Unité de Recherche: Génétique, Biodiversité et Valorisation des Bioressources UR/09-30, Institut Supérieur de Biotechnologie de Monastir, Monastir, Tunisia aut NATIONALLICENCE 700 1- Ben Slimane Badreddine Institut Supérieur des Sciences et Technologies de l'Environnement de Borj Essedria, Borj Essedria, Tunisia aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/5(2011-11-01), 1429-1437 0932-0113 109:5<1429 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer