caa a22 4500 445341130 CHVBK 20180317142813.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00436-011-2416-2 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2416-2 In vivo anthelmintic activity of chelidonine from Chelidonium majus L. against Dactylogyrus intermedius in Carassius auratus [Elektronische Daten] [Jia-Yun Yao, Zhi-Ming Zhou, Xiao-yi Pan, Gui-jie Hao, Xi-Lian Li, Yang Xu, Jin-Yu Shen, Hong-shun Ru, Wen-lin Yin] Dactylogyrus intermedius is one of the most common and serious cause of parasitic diseases of freshwater fish in aquaculture, and can cause morbidity and high mortality in most species of freshwater fish worldwide. To attempt controlling this parasite and explore novel potential antiparasitic agents, the present study was designed to ascertain the anthelmintic activity of Chelidonium majus L. whole plant and to isolate and characterize the active constituents against D. intermedius. The ethanol extract from C. majus whole plant showed significant anthelmintic activity against D. intermedius [EC50 (median effective concentration) value = 71.5mgL−1] and therefore subjected to further isolation and purification using various chromatographic techniques. A quaternary benzo[c]phenanthridine alkaloid exhibited significant activity against D. intermedius was obtained and identified as chelidonine. In vivo anthelmintic efficacy tests exhibited that chelidonine was 100% effective against D. intermedius at a concentration of 0.9mgL−1, with EC50 value of 0.48mgL−1 after 48h of exposure, which is more effective than the positive control, mebendazole (EC50 value = 1.3mgL−1). In addition, the 48-h median lethal concentration (LC50) for chelidonine against the host (Carassius auratus) was 4.54mgL−1. The resulting therapeutic index for chelidonine was 9.46. These results provided evidence that chelidonine might be potential sources of new antiparasitic drugs for the control of Dactylogyrus. Springer-Verlag, 2011 Yao Jia-Yun Institute of Freshwater Fisheries of Zhejiang, 313001, Huzhou, Zhejiang, China aut Zhou Zhi-Ming Institute of Freshwater Fisheries of Zhejiang, 313001, Huzhou, Zhejiang, China aut Pan Xiao-yi Institute of Freshwater Fisheries of Zhejiang, 313001, Huzhou, Zhejiang, China aut Hao Gui-jie Institute of Freshwater Fisheries of Zhejiang, 313001, Huzhou, Zhejiang, China aut Li Xi-Lian College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, Shaanxi, China aut Xu Yang Institute of Freshwater Fisheries of Zhejiang, 313001, Huzhou, Zhejiang, China aut Shen Jin-Yu Institute of Freshwater Fisheries of Zhejiang, 313001, Huzhou, Zhejiang, China aut Ru Hong-shun Institute of Freshwater Fisheries of Zhejiang, 313001, Huzhou, Zhejiang, China aut Yin Wen-lin Institute of Freshwater Fisheries of Zhejiang, 313001, Huzhou, Zhejiang, China aut Parasitology Research Springer-Verlag 109/5(2011-11-01), 1465-1469 0932-0113 109:5<1465 2011 109 436 https://doi.org/10.1007/s00436-011-2416-2 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2416-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yao Jia-Yun Institute of Freshwater Fisheries of Zhejiang, 313001, Huzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Zhou Zhi-Ming Institute of Freshwater Fisheries of Zhejiang, 313001, Huzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Pan Xiao-yi Institute of Freshwater Fisheries of Zhejiang, 313001, Huzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Hao Gui-jie Institute of Freshwater Fisheries of Zhejiang, 313001, Huzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Li Xi-Lian College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, Shaanxi, China aut NATIONALLICENCE 700 1- Xu Yang Institute of Freshwater Fisheries of Zhejiang, 313001, Huzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Shen Jin-Yu Institute of Freshwater Fisheries of Zhejiang, 313001, Huzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Ru Hong-shun Institute of Freshwater Fisheries of Zhejiang, 313001, Huzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Yin Wen-lin Institute of Freshwater Fisheries of Zhejiang, 313001, Huzhou, Zhejiang, China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/5(2011-11-01), 1465-1469 0932-0113 109:5<1465 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer