caa a22 4500 445341157 CHVBK 20180317142813.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00436-011-2392-6 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2392-6 Visualization of chromosomes in the binucleate intestinal parasite Giardia lamblia [Elektronische Daten] [Hai Shen, Lei Cao, Ji Li, Xi Tian, Zhi Yang, Yue Wang, Yu Tian, Si Lu] Mitosis of Giardia lamblia is a complex and rapid event that is poorly understood at the cellular and molecular levels. Therefore, we conducted this study to determine (1) whether the two nuclei have similar or different chromosomes, (2) the number of chromosomes of G. lamblia, and (3) the morphology and karyotype of the chromosomes. Trophozoites of the C2 and WB strains of G. lamblia were grown in modified TYI-S-33 medium at 37°C. The trophozoites were collected, and sample slides were prepared for conventional light and scanning electron microscopy. Light microscopy revealed five pairs of chromosomes. The chromosomes were approximately 0.64-0.94μm long with a short rod-like shape and were usually arranged in pairs. Scanning electron microscopy yielded similar findings, and 10 chromosomes could be seen in each nucleus. Thus, the chromosome number of G. lamblia is 2n = 10. Chromosomes in pair 1 are submetacentric chromosomes, while pairs 2-5 are telocentric chromosomes. The present study shows that G. lamblia trophozoites have typical condensed chromosomes during mitosis and contains five pairs of chromosomes. The karyogram shows good fit to the formula2n = 10 = 2sm + 8t revealed by scanning electron microscopy. Springer-Verlag, 2011 Shen Hai School of Biological Sciences, Hebei United University, 063000, Tangshan, China aut Cao Lei School of Biological Sciences, Hebei United University, 063000, Tangshan, China aut Li Ji School of Biological Sciences, Hebei United University, 063000, Tangshan, China aut Tian Xi School of Biological Sciences, Hebei United University, 063000, Tangshan, China aut Yang Zhi Department of Pathogenic Biology, Vocational and Technical College of Tangshan, 063000, Tangshan, China aut Wang Yue Department of Pathogenic Biology, Vocational and Technical College of Tangshan, 063000, Tangshan, China aut Tian Yu Department of Pathogenic Biology, Vocational and Technical College of Tangshan, 063000, Tangshan, China aut Lu Si Department of Parasitology, Capital Medical University, 100069, Beijing, China aut Parasitology Research Springer-Verlag 109/5(2011-11-01), 1439-1445 0932-0113 109:5<1439 2011 109 436 https://doi.org/10.1007/s00436-011-2392-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2392-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shen Hai School of Biological Sciences, Hebei United University, 063000, Tangshan, China aut NATIONALLICENCE 700 1- Cao Lei School of Biological Sciences, Hebei United University, 063000, Tangshan, China aut NATIONALLICENCE 700 1- Li Ji School of Biological Sciences, Hebei United University, 063000, Tangshan, China aut NATIONALLICENCE 700 1- Tian Xi School of Biological Sciences, Hebei United University, 063000, Tangshan, China aut NATIONALLICENCE 700 1- Yang Zhi Department of Pathogenic Biology, Vocational and Technical College of Tangshan, 063000, Tangshan, China aut NATIONALLICENCE 700 1- Wang Yue Department of Pathogenic Biology, Vocational and Technical College of Tangshan, 063000, Tangshan, China aut NATIONALLICENCE 700 1- Tian Yu Department of Pathogenic Biology, Vocational and Technical College of Tangshan, 063000, Tangshan, China aut NATIONALLICENCE 700 1- Lu Si Department of Parasitology, Capital Medical University, 100069, Beijing, China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/5(2011-11-01), 1439-1445 0932-0113 109:5<1439 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer