caa a22 4500 445341211 CHVBK 20180317142813.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00436-011-2371-y doi (NATIONALLICENCE)springer-10.1007/s00436-011-2371-y Toxocara canis larvae reinfecting BALB/c mice exhibit accelerated speed of migration to the host CNS [Elektronische Daten] [Petra Kolbeková, David Větvička, Jan Svoboda, Karl Skírnisson, Markéta Leissová, Martin Syrůček, Helena Marečková, Libuše Kolářová] Using a small animal imaging system, migratory activity of Toxocara canis larvae stained by carboxyfluorescein succinimidyl ester (CFSE) was observed post primary infection (PPI) and post reinfection (PR) of BALB/c mice. Each infection was performed with 1,000 larvae per mouse. Primary infections were performed with labeled larvae, while for challenge infections the reinfecting larvae were stained by CFSE. The worm burden in mouse organs was determined during a period from 6h to 21days and 4months PPI and PR. In comparison with primary infections that led to the first larvae appearance in the brain after 60h, greatly accelerated migration of the parasites administered 3weeks PPI to the CNS and eyes of challenged mice was noted—in both organs the larvae appeared 6h PR. In all challenged mice, reinfecting larvae prevailed in the resident parasite population. Preliminary experiments with Toxocara cati larvae also revealed early brain involvement in primarily infected mice. Staining of T. canis larvae by CFSE had no effect on the development of a humoral antibody response against T. canis excretory-secretory antigens. In ELISA, elevated levels of specific IgG and IgG1 were noted on day14 PPI and the levels of antibodies increased till the end of experiment. Reinfection induced an increase in the levels of both antibodies. In terms of optical density, IgG1 antibodies gave higher values in all sera examined. In ELISA for IgG antibodies, an increase in the avidity index of around 50% was detected 1month PPI; higher-avidity antibodies were also detected in sera of reinfected animals. Springer-Verlag, 2011 Kolbeková Petra National Reference Laboratory for Tissue Helminthoses, General University Hospital in Prague, Prague, Czech Republic aut Větvička David Department of Immunology and Gnotobiology, Institute of Microbiology, Academy of Science, Prague, Czech Republic aut Svoboda Jan Department of Immunology and Gnotobiology, Institute of Microbiology, Academy of Science, Prague, Czech Republic aut Skírnisson Karl Institute of Experimental Pathology, University of Iceland, Keldur, Iceland aut Leissová Markéta National Reference Laboratory for Tissue Helminthoses, General University Hospital in Prague, Prague, Czech Republic aut Syrůček Martin Department of Pathology, Na Homolce Hospital, Prague, Czech Republic aut Marečková Helena Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Studničkova 7, 128 00, Prague 2, Czech Republic aut Kolářová Libuše National Reference Laboratory for Tissue Helminthoses, General University Hospital in Prague, Prague, Czech Republic aut Parasitology Research Springer-Verlag 109/5(2011-11-01), 1267-1278 0932-0113 109:5<1267 2011 109 436 https://doi.org/10.1007/s00436-011-2371-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2371-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kolbeková Petra National Reference Laboratory for Tissue Helminthoses, General University Hospital in Prague, Prague, Czech Republic aut NATIONALLICENCE 700 1- Větvička David Department of Immunology and Gnotobiology, Institute of Microbiology, Academy of Science, Prague, Czech Republic aut NATIONALLICENCE 700 1- Svoboda Jan Department of Immunology and Gnotobiology, Institute of Microbiology, Academy of Science, Prague, Czech Republic aut NATIONALLICENCE 700 1- Skírnisson Karl Institute of Experimental Pathology, University of Iceland, Keldur, Iceland aut NATIONALLICENCE 700 1- Leissová Markéta National Reference Laboratory for Tissue Helminthoses, General University Hospital in Prague, Prague, Czech Republic aut NATIONALLICENCE 700 1- Syrůček Martin Department of Pathology, Na Homolce Hospital, Prague, Czech Republic aut NATIONALLICENCE 700 1- Marečková Helena Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Studničkova 7, 128 00, Prague 2, Czech Republic aut NATIONALLICENCE 700 1- Kolářová Libuše National Reference Laboratory for Tissue Helminthoses, General University Hospital in Prague, Prague, Czech Republic aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/5(2011-11-01), 1267-1278 0932-0113 109:5<1267 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer