caa a22 4500 44534122X CHVBK 20180317142813.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00436-011-2386-4 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2386-4 Scanning electron microscopy of the human low-density lipoprotein interaction with the tegument of Schistosoma mansoni [Elektronische Daten] [Adriana Pereira, Rafael Padilha, José Lima-Filho, Maria Chaves] The interaction between host molecules and Schistosoma mansoni has been regarded as a key feature for parasite survival. In this work, scanning electron microscopy was used to study the interaction of human low-density lipoprotein (LDL) with the tegument of the adult worm of S. mansoni. Worms were incubated in RPMI 1640 containing 10% of LPDS and 40μgLDL/mL during 30, 60, and 120min. Control worms were processed in the same way, without LDL. After the incubations, the samples were fixed and processed to scanning electron microscopy. The results demonstrated interaction of the LDL particles with the male parasite tegument. Male and female worms incubated without LDL from 0 (control) to 120min did not show alterations in the tegument. It was observed a larger number of LDL particles on the dorsal region of male adult worm than others regions (anterior, posterior and gynecophoral canal). The female tegument did not show adherence of LDL. Aggregates on the tegument of the male worm were in greater number and size in the incubation times of 30 and 60min than 120min. The comparison between 30 and 120min of incubation showed that the particles' size diminished from 2,650-860nm to 634-363nm, respectively. Such reduction can be due to the capture and the use of the lipids by the worm. Therefore, the internalization of lipids from LDL by the male worms seems to be a mechanism independent of endocytosis. Differences between males and females suggest lipid transference from male to female through gynecophoral canal. Springer-Verlag, 2011 Pereira Adriana Laboratório de Imunopatologia Prof. Keizo Asami (LIKA), Universidade Federal de Pernambuco, Avenida Professor Moraes Rego, s/n—CEP 50670-901, Recife, Pernambuco, Brazil aut Padilha Rafael Laboratório de Imunopatologia Prof. Keizo Asami (LIKA), Universidade Federal de Pernambuco, Avenida Professor Moraes Rego, s/n—CEP 50670-901, Recife, Pernambuco, Brazil aut Lima-Filho José Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil aut Chaves Maria Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil aut Parasitology Research Springer-Verlag 109/5(2011-11-01), 1395-1402 0932-0113 109:5<1395 2011 109 436 https://doi.org/10.1007/s00436-011-2386-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2386-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pereira Adriana Laboratório de Imunopatologia Prof. Keizo Asami (LIKA), Universidade Federal de Pernambuco, Avenida Professor Moraes Rego, s/n—CEP 50670-901, Recife, Pernambuco, Brazil aut NATIONALLICENCE 700 1- Padilha Rafael Laboratório de Imunopatologia Prof. Keizo Asami (LIKA), Universidade Federal de Pernambuco, Avenida Professor Moraes Rego, s/n—CEP 50670-901, Recife, Pernambuco, Brazil aut NATIONALLICENCE 700 1- Lima-Filho José Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil aut NATIONALLICENCE 700 1- Chaves Maria Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/5(2011-11-01), 1395-1402 0932-0113 109:5<1395 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer